摘要
通过构建肌肉组织cDNA文库分离到鳜肌球蛋白轻链2基因(MLC2),基因登录号为FJ428249.MLC2基因cDNA序列全长1206bp,编码区长度为579bp.MLC2基因开放阅读框编码170个氨基酸,具有EF-手相家族蛋白全部4个EF-手相结构.与已报道的其他动物MLC2相比较,所推导氨基酸序列同源性在66.3%～97.6%,其中与Ca2+结合区域非常保守,7种鱼氨基酸序列同源性为100%.采用实时荧光定量PCR方法对鳜MLC2发育表达分析表明,MLC2在原肠期开始有低量表达,与原肠期相比,尾芽期、肌肉效应期和仔鱼阶段MLC2表达量随发育阶段渐进而升高.
The myosin light chain 2（MLC2） gene was isolated from the muscle tissue cDNA library of Siniperca chuasti. The length of the MLC2 cDNA is 1 206 bp, and the open reading frame is 579 bp. The MLC2 encodes 170 amino acids, which includes four EF-hand structures. Compared with the MLC2 of other animals reported, the homology of the deduced amino acids sequences was between 66.3% -97.6%. The primary structures of the Ca^2＋ - binding domain were well conserved among the MLC2s of seven other fish species. The ontogenetic expression analysis by real-time PCR showed that the MLC2 mRNA was first detected in gastrula stage and its expression gradually increased from the tail bud stage to larval stage.
出处
《湖南师范大学自然科学学报》
CAS
北大核心
2009年第3期78-83,共6页
Journal of Natural Science of Hunan Normal University
基金
国家自然科学基金资助项目(30771644)
湖南省自然科学基金资助项目(K082014-13)
湖南省教育厅资助项目(08C133)
关键词
鳜
肌球蛋白轻链2
CDNA克隆
发育表达
Siniperca chuasti
myosin light chain 2
cDNA cloning
gene expression
