摘要
目的探索以小剂量豚鼠脊髓匀浆为抗原,建立Wistar大鼠实验性自身免疫性脑脊髓炎(EAE)模型,并观察其病理改变。方法按脊髓湿重与冰盐水体积为1:10的比例制备豚鼠脊髓匀浆抗原,免疫Wistar大鼠,建立EAE模型;留取脑和脊髓进行HE染色及三色染色,光镜下观察病理改变。结果Wistar大鼠免疫后第17.20±6.48天第一次发病,发病形式多样,除表现EAE常见症状外,尚出现痉挛状态、斜颈等特殊症状。HE染色发现神经组织内炎细胞浸润,血管"袖套"样病灶形成;三色染色可见轴突肿胀,呈串珠状,且不连续,着色不均匀;髓鞘结构层次紊乱,疏松,崩解。结论小剂量脊髓匀浆诱导的EAE模型,病程及发病表现更接近于人类多发性硬化的表现,且经济实用,是研究多发性硬化的理想动物模型。
Objective To establish a kind of experimental autoimmune encephalomyelitis model by Low-dose guinea pig spinal cord homogenate（ GPSCH ） in Wistar rats and observe the pathological changes. Methods Wistar rats were immunized with complete Freund＇s adjuvant （CFA） and GPSCH,in which the proportion of weight of spinal cord and volume of icy salt water was 1 ： 10. The pathological changes of brain and spinal cord tissue sections were observed under light microscopy after HE staining and trichrome staining. Results Wistar rats showed disorders 17.20 ± 6.48 days after immunization with different modalities. The clinical symptoms included spasticity,head tilt et al,beside the classic symptoms of EAE. There were infiltration of inflannnatory cells inside nerve tissue and perivascular cuffings in HE staining. In trichrome staining,the axon with inhomogeneous chromatosis was swollen and beaded-discontinued;The myelin was porous and partly disaggregated. Conclusion The low-dose GPSCH indueed EAE model, showing almost the same clinical symptoms and course as muhiple selerosis,was an ideal animal model for study of muhiple sclerosis.
出处
《脑与神经疾病杂志》
2009年第5期367-369,共3页
Journal of Brain and Nervous Diseases
基金
河北省科技攻关项目(22761147)
关键词
自身免疫性
脑脊髓炎
多发性硬化
动物模型
三色染色
Aotoimmunity
Encephalomyelitis
Multiple sclerosis
Animal model
Triehrome staining
