摘要
应用随机扩增多态性DNA(RAPD)技术,对9个不同地理种群美国白蛾进行了遗传多样性和UPMGA聚类分析。结果表明:25个RAPD引物共扩增191个位点,片段大小在200～2000bp,其中158个是多态位点,多态百分率为82.72%,遗传距离指数在0.2043～0.5108,遗传相似性系数在0.4892～0.7957;9个不同地理种群的美国白蛾可以分成3类:辽宁省和山东类群、北京和天津类群、美国类群,表明美国白蛾不同地理种群间的遗传分化与地理位置相关。
The genetic diversity and UPMGA clustering of nine Hyphantria cunea geographic＇ populations were analyzed by random amplified polymorphic DNA （RAPD） technique. A total of 191 bands were obtained from 25 effective primers, of which, 158 bands were polymorphic. accounting for 82. 72%. The genetic distance index of the populations was 0. 2043-0. 5108, and the similarity coefficient was 0. 4892-0. 7957. The nine populations could be divided into three groups, i. e. , Liaoning and Shandong group, Beijing and Tianjing group, and American group, suggesting that the genetic differentiation of H. cunea populations was correlated with geographical location.
出处
《生态学杂志》
CAS
CSCD
北大核心
2009年第10期2032-2036,共5页
Chinese Journal of Ecology
基金
北京市植物保护站专项基金资助项目(D0706003050164-2007-3)
关键词
美国白蛾
RAPD
遗传分化
Hyphantria cunea
RAPD
genetic differentiation
