摘要
豆卷叶螟是南京地区的主要食叶性害虫。以抗性亲本溧水中子黄豆和感性亲本南农493-1杂交组合正交F2群体为材料,在田间自然虫源条件下F2单株叶片损失率为抗性指标,利用已构建的SSR分子标记图谱和Windows QTL Cartographer V2.5软件包的复合区间作图法和多区间作图法,定位大豆对豆卷叶螟抗性的QTL。结果表明:利用复合区间作图法检测到位于D1b和K连锁群上的2个QTL;利用多区间作图法则检测到位于A2、D1b、K和N连锁群上的4个QTL和6个互作QTL;其中有两个共同的QTL,至少解释表型变异的19.2%。这些结果为抗性性状的遗传剖析和标记辅助育种提供理论依据。
The bean pyralid （Lamprosema indicata Fabricius） is one of the main leaf- feeding insect species in Nanjing area. Plants of F2 population derived from a direct cross between Lishuizhongzihuang （resistance） and Nannong 493 - 1 （susceptible） were measured for resistance in soybean to natural population of bean pyralid in the field according to its defoliation percentage in 2006. Quantitative trait loci （QTL） detection for the resistance was carried out by using a direct F2 population. The results showed that two QTLs were mapped on the linkage groups Dlb and K by using composite interval mapping method. Four QTLs along with six interactions were identified in the linkage groups A2, D1b, K and N by using multiple interval mapping method. Among these QTLs, two common QTLs explained more than 19.2% of total phenotypic variance. The results presented will enhance the understanding of genetic basis of the resistance to bean pyralid and provide a theoretical foundation for soybean markerassisted selection.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2009年第3期365-369,共5页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金项目(30470998)
教育部高校博士点基金项目(20060307008)
新世纪优秀人才支持计划(NCET-05-0489)
“111”项目(B08025)
农业部项目(M200814)
江苏省自然科学基金项目(BK2008335)
关键词
豆卷叶螟
QTL
大豆
多区间作图
Bean pyralid
Quantitative trait loci
Soybean
Multiple interval mapping
