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大鼠感染性脑损伤中神经细胞内游离钙的变化

Alterations of intracellular free calcium concentration of cerebralneuronal cells in infectious brain injury in rats
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摘要 采用荧光法测定负载Fura-2/AM大脑皮层突触体内游离钙[Ca2+]i浓度在大鼠感染性脑损伤(感脑)中的变化及其意义。结果显示:百日咳菌液组(0.5h,4h,24h)的[Ca2+]i,含水量(WC)及伊文思蓝(EB)含量均高于对应盐水对照组(P<0.05或<0.01);菌液组[Ca2+]i增高呈时效关系,以24h组含量最高(4hvs0.5h,P<0.05;24hvs4h,P<0.01),并分别与WC及EB含量呈高度正相关(0.5h,4h,P<0.01)及无相关(24h,P>0.05)。结果表明。 Intracellular free calcium concentration, [Ca^(2+)]i, of cerebral synaptosomes loaded by Fura2/AM was measured by fluorescent method to clarify alterations of [Ca^(2+)]i in infectious brain injury (IBI) in rats. The results showed that [Ca^(2+)]i, water content (WC), and Evans blue content (EB) in groups injected by Bordetella pertussis suspension (BP) were increased compared to those in groups injected by normal saline (NS) at 0.5h, 4h, and 24h, respectively. Increased [Ca^(2+)]i in BP groups showed time-dependent relationship and the highest level in the 24h group. There was positive correlation (0.5h, 4h) and no correlation (24h) between [Ca^(2+)]i and WC or EB. It is suggested that [Ca^(2+)]i accumulation of cerebral neuronal cells plays an important role in the pathogenetic mechanism of the acute and delayed IBI induced by BP.
出处 《湖南医科大学学报》 CSCD 1998年第4期353-355,共3页 Bulletin of Hunan Medical University
基金 国家自然科学基金
关键词 颅脑损伤 神经细胞 动物模型 游离钙 Bordetella pertussis  infection brain injuries cerebral cortex intracellular free calcium concentration* synaptosomes Fura2/AM* disease model,animal rats
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