摘要
目的探讨ER PvuⅡ基因多态性与儿童氟斑牙的关系。方法选取河南省开封、通许两县高氟区和对照区的8-12岁儿童作为观察对象;采用氟离子选择电极法检测尿氟含量,利用PCR-RFLP技术分析ER PvuⅡ基因多态性。结果高氟区和对照区儿童氟斑牙患病率分别为51.7%和0%,其差异有统计学意义（P〈0.05）。高氟区ER PvuⅡ基因型分布频率：氟斑牙患者PP 20.3%（15/74）、Pp 36.5%（27/74）、pp 43.2%（32/74）;非患者PP 11.6%（8/69）、Pp 46.4%（32/69）、pp 42.0%（29/69）。对照区PP 23.4%（22/94）、Pp 47.9%（45/94）、pp 28.7%（27/94）;三组基因型频率分布差异无统计学意义（P〉0.05）。结论高氟区儿童ER PvuⅡ基因型与氟斑牙发生无明显关系。
Objective To investigate the relationship between ER gene Pvu Ⅱ polymorphism and dental fluorosis. Methods Children aged 8-12 years old who lived in high fluoride and control areas in Kaifeng and Tongxu counties were recruited. Fluoride concentration in urine was detected by using of fluoride ion - selective electrode method. ER gene Pvu Ⅱ polymorphism was analyzed using PCR - RFLP method. Results C, enotype of ER Pvu H were PP 20.3 % （ 15/74 ）, Pp 36.5 % （ 27/74 ）, pp 42.7 % （ 32/74 ） in children with dental fluorosis; PP 11.6 % （8/69） , Pp 46.4 % （32/69） , pp 46.2 % （29/69） in controls from high fluoride areas ; and PP 23.4 % （22/94）, Pp 47.9 % （45/94） , pp 28.7 % （27/94） in children from control areas. There was no significant difference among these three groups （ P 〉 0.05 ）. Conclusion There was no correlation between ER Pvu Ⅱ polymorphism and dental fluorosis.
出处
《河南预防医学杂志》
2009年第5期321-323,329,共4页
Henan Journal of Preventive Medicine
基金
河南省教育厅自然基金资助项目(2009A330005)
郑州市科技攻关项目(X2008HH0454)
关键词
氟斑牙
基因多态性
雌激素受体
dental fluorosis
gene polymorphism
estrogen receptor
