摘要
目的探讨肾脏病患儿尿核心蛋白聚糖(decorin)水平与肾脏病理损伤程度的关系。方法选择2006年9月-2007年12月行肾活检的肾脏病患儿49例。收集患儿的临床指标及24 h尿液,采用ELISA法测定其尿decorin水平。肾脏病理损伤采用Katafuchi半定量积分的标准,根据肾损伤积分分为3组:肾病理损伤轻微26例(Ⅰ组);肾病理中度损伤16例(Ⅱ组);肾病理重度损伤7例(Ⅲ组)。应用SPSS11.5软件进行统计学分析。结果患儿尿decorin水平[Ⅰ组:(5.624±0.889)μg/L,Ⅱ组:(33.006±3.568)μg/L,Ⅲ组:(76.800±9.012)μg/L],随肾损伤程度的增加而显著增加(P<0.01)。3组患儿尿微量清蛋白比较无显著性差异(P>0.01)。Ⅲ组患儿尿β2-微球蛋白(β2-MG)水平[(2 034±668)μg/L]与Ⅰ、Ⅱ组[(769±213)、(656±260)μg/L]比较差异显著(P<0.05)。尿decorin水平与肾损伤程度呈显著正相关(r=0.937 P<0.01),尿decorin水平与肾小管损伤程度呈显著正相关(r=0.909 P<0.01),尿decorin水平与肌酐清除率呈显著负相关(r=-0.415 P<0.01),尿β2-MG水平与肾小管损伤程度呈正相关(r=0.347 P<0.05)。结论尿decorin水平可间接判定肾脏病理损伤的轻重。随着肾脏病理损伤程度的增加,尿decorin水平亦升高。检测尿decorin水平可作为判断肾脏病理损伤程度的临床指标之一。
Objective To explore the excretion of urine decorin in children with kidney disease and its correlation with the renal damage. Methods Forty - nine children who were admitted into hospital with kidney disease from Sep. 2006 to Dec. 2007 were investigated. Twenty- four hours urine and clinical parameters were collected. Urine decorin was detected by enzyme linked immunosorbent assay （ELISA）. Katafuchi semi- quantitative scoring system was used to evaluate the renal damage. The children were classified into 3 groups according to the renal damage：group Ⅰ including 26 cases with mild damage ;group Ⅱ including 16 cases with moderate damage;and group Ⅲ including 7 cases with severe damage. SPSS 11.5 software was used to analyze the data. Results The levels of urine decorin were ： （5. 624 ± 0. 889） μg/L in group Ⅰ , （33. 006 ±3. 568）μg/L in group Ⅱ ,and （76. 800 ±9. 012）μg/L in group Ⅲ ,respectively. The levels of urine decorin were increased with the severity of renal damage （ P 〈 0.01 ）. There was no difference of urine albumin excretion among 3 groups （ P 〉 0.01 ）. The levels of urine 132 - microglobulin （ β2 - MG） were （769± 213 ） μg/L in group Ⅰ, （656 ± 260 ）μg/L in group Ⅱ , （ 2 034 ± 668 ） μg/L in group Ⅲ, respectively. There was significant difference among 3 groups （ P 〈 0.05 ）. The levels of urine decorin were positively correlated with renal damage（r =0.937 P〈0.01） and renal tubule damage （r=0.909 P〈0.01）. A negative correlation was found between the levels of urine decorin and the blood creatinine clearance rate（ r = 0. 415 P 〈 0.01 ）. The levels of urine β2 - MG were positively correlated with renal damage （ r = 0. 347 P 〈 0.05 ）. Conclusions The levels of urine decorin can act as the clinical marker of renal damage. With the severity of renal lesions, the levels of urine decorin were increased.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2009年第17期1339-1340,共2页
Journal of Applied Clinical Pediatrics
基金
南京市科技局人才培养项目资助(200306062)
