摘要
目的:通过检测血管紧张素Ⅱ(AngⅡ)诱导的肥大心肌细胞总蛋白含量变化,说明降压通脉方对肥大心肌细胞的影响。方法:培养心肌细胞,建立AngⅡ诱导心肌细胞肥大模型,运用考马斯亮蓝法检查心肌细胞总蛋白含量。结果:AngⅡ可显著增加心肌细胞总蛋白含量的表达(P<0.05),高浓度的降压通脉方有心肌细胞毒性,降压通脉方能减少肥大心肌细胞的总蛋白含量,与模型组相比有统计学差异(P<0.05)。结论:降压通脉方能抑制AngⅡ诱导的肥大心肌细胞总蛋白表达,具有防止心肌细胞肥大的作用,从而防治左室肥厚。
The experimental research is adding angiotensin Ⅱ to the original cultured myocardial cells and giving western or chinese medicine to intervention.Methods：Adding angiotensin Ⅱ to the original cultured myocardial cells.To detect the difference in total protein content of blank group,model group,the Western Medicine group,Chinese medicine groups by Coomassie Brilliant Comparison.Results：High concentrations fo Jiangya Tongmai Decoction have myocardial cytotoxicity.Ang Ⅱ on cultured neonatal rat cardiomyocytes cells significantly promote the role of mast. Jiangya Tongmai Decoction can decrease total protein content of cardiac myocyte. Conclusion ：Jiangya Tongmai Decoction can inhibit the Ang Ⅱ-induced myocardial hypertrophy.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2009年第8期584-586,共3页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家自然科学基金资助项目(30572460)
