摘要
目的:探讨补髓生血颗粒对慢性再生障碍性贫血(CAA)患者骨髓单个核细胞c-kit mRNA及CD117表达的影响。方法:分别采用逆转录-聚合酶链反应(RT-PCR)法及流式细胞技术检测CAA病人治疗前后骨髓单个核细胞c-kit mRNA及CD117水平的变化。结果:CAA患者骨髓c-kit mRNA及其CD117表达水平高于正常对照组,治疗后二者表达水平均有不同程度下降,并且补髓生血颗粒对二者表达水平的下调程度优于再障生血片对照组(P<0.05)。结论:CAA患者骨髓c-kit mRNA及CD117的表达存在异常;补髓生血颗粒剂可能通过调节骨髓c-kit mRNA及CD117的表达来改善CAA的骨髓造血功能,促进骨髓造血功能恢复。
Objective： To discuss the clinical effective of Busuishengxue granules to chronic aplastic anemia （CAA） patients, and to observe the difference in expression level of myelo-cytokine c-kit/CD117 before and after treatment. Methods： This experiment employed reverse transcription-polymerase chain reaction （RT-PCR） method to investigate the change of expression level of bone marrow mononuclear cell （BMMC） c-kit receptor mRNA and its c-kit receptor protein CD117 with flow cytometer method （FCM）. Results：The expression level of BMMC c-kit receptor mRNA and its receptor protein CD117 in CAA patients were higher than that in normal control group. And after treatment both of the expression levels descended to different degree. Moreover, the descent in Busuishengxue Granule group was superior to that in zaizhangshengxue Tablet group. Conclusions： There was abnormal expression of bone marrow SCF receptor ckit/CD117 in CAA patients. Busuishengxue Granules by regulating the bone marrow c-kitmRNA and the expression of CD117 improved the hematopoietic function of bone marrow in CAA.
出处
《中国实验方剂学杂志》
CAS
北大核心
2009年第9期49-51,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金(30271621)
