摘要
通过探索不同的裂解液成分配比和优化2-DE条件,建立了适合胡杨叶蛋白质组学研究的技术体系,同时以同一小枝上的异形叶为试验材料寻找异形叶间差异表达的蛋白质。结果表明:使用含7mol/L尿素、2mol/L硫脲、2%CHAPS、60mmol/LDTT、0.2%载体两性电解质的裂解液复溶蛋白沉淀的效果好。通过对差异蛋白点的串联质谱鉴定,发现异形叶在光合及呼吸作用方面存在差异。本研究为了解异形叶产生的分子调控机制,揭示胡杨的耐逆性积累了有价值的信息。
By exploring the different components of the lysis buffer and optimize the 2-DE conditions, established the best proteomics technical system for Populus euphratica＇ s heteromorphic leaves, while take the heteromorphic leaves in the same blanche as the test materials to find differences between the protein expressions of the leaves. It showed that the lysis solution which containing 2mmol/L thiourea,7mmol/L urea, 2% CHAPS, 60mmol/L DTT and 0.2% IPG buffer could dissolve the protein better. Through tandem mass spectrum, the results show that heteromorphic leaves are different in photosynthesis and respiration. This research offered valuable informations for understanding the molecular mechanism during leaves development and elucidating the mechanism of the eco-adaptability of Populus euphratica.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2009年第9期40-44,共5页
China Biotechnology
关键词
胡杨
异形叶
蛋白质纽学
Populus euphratica Oliv Heteromorphic leaves Proteomics
