摘要
目的观察骨调素（ＯＰＮ）在大鼠实验性新月体肾炎肾脏组织中的表达及功能性抑制ＯＰＮ的活性对巨噬细胞在肾脏局部浸润的影响。方法ＯＰＮ在肾脏组织蛋白和基因水平的表达分别应用免疫组化双标记及原位杂交技术。结果在实验性新月体肾炎肾脏组织内ＯＰＮ的表达明显升高，并与肾脏局部巨噬细胞的浸润、肾脏病理损害程度及肾功能的下降明显相关。应用抗ＯＰＮ抗体治疗以后可显著抑制ＯＰＮ在肾脏组织的表达，同时巨噬细胞在肾脏组织局部的浸润也明显减少，肾脏病理损害减轻、肾功能明显改善。结论ＯＰＮ的高表达是实验性新月体肾炎发病过程中巨噬细胞在肾脏组织局部浸润的重要介导因子，抑制ＯＰＮ的表达和功能可显著抑制肾脏局部巨噬细胞的聚集及改善肾功能。
Objective To investigate the role of osteopontin in the accumulation of macrophages in rat experimental crescentic glomerulonephritis.Methods Double labelling immunostaining and in situ hybridization.Results Osteopontin expression was highly increased in the glomerular and tubulointerstitial area,and closely correlated with the proteinuria and damaged renal function,especially macrophage infiltration.AntiOPN treatment significantly inhibited the macrophages infiltration and glomerular injury and prevented a loss of renal function.Conclusion OPN plays an important role in inducing macrophage infiltration and accumulation in a rat model of crescentic glomerulonephritis.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
1998年第4期220-224,共5页
Chinese Journal of Nephrology
基金
广东省科委重点项目基金
澳大利亚肾脏病学会基金
关键词
骨调素
巨噬细胞
新月体肾炎
肾炎
Osteopontin Macrophage Crescentic glomerulonephritis
