摘要
目的提高完善感冒丸的质量标准。方法采用显微鉴别处方中的桑叶、薄荷、甘草。采用TLC法对处方中麻黄、桔梗进行定性鉴别;并用HPLC法对制剂中黄芩有效成分黄芩苷进行含量测定,色谱柱：广州菲罗门luna C18;流动相：甲醇-0.4%磷酸溶液（47∶53）;流速：1mL.min-1,柱温：30℃,检测波长：280nm。结果HPLC法测定黄芩苷在0.2042~2.042μg范围内有良好线性关系,r=0.9999。平均回收率为96.7%,RSD为1.06%（n=6）。结论定性、定量方法简便、可靠、准确、专属性强,可用于感冒丸的质量控制。
Objective To improve the quality standard for Ganmao wan. Methods The presence of Radix Platycodonis, Flos Lonicerae Japonicae, Radix Peucedani and Herba Ephedrae was identified by TLC. The content of Radix Scutellariaβ-D-Gluopyranosiduronic acid, 5, 6-dihydroxy-4-oxo-2-phenyl-4H-1- benzopyran-7-yl was determined by HPLC. Guangdong phenomenex C18 column was adoped. The mobile phase consisted of methanol -0.4%phosphoric acid（47 ： 53） and the detection wavelength was 280nm. The column temperature was 30℃. The flow rate was 1mL · min^-1. Results The good linear ranges of baicalin was 0. 2042-2. 042μg （r= 0. 9999）. The average recovery was 96.7% （n = 6） and RSD was 1.06%. Conclusions This method is simple, accurate and reliable. It can be used as a quantitative determination method for the quality control of Ganmao Wan.
出处
《中国药事》
CAS
2009年第9期878-880,共3页
Chinese Pharmaceutical Affairs
基金
国家药品标准提高行动计划(编号国药典发[2005]167号)
