摘要
目的:通过基因治疗与温热治疗方法相结合,观测两者治疗手段是否有协同作用。方法:将40只荷瘤小鼠随机分为4组,A组:E1B-55KD基因缺陷腺病毒感染组;B组:温热治疗组;C组:E1B-55KD基因缺陷腺病毒感染+温热治疗组;D组:空白对照组。治疗前后测量瘤体直径,实验前后取瘤体称重;Western蛋白分析对HSP进行定位、定量检测;流式细胞仪进行T细胞亚群检测,计算CD3+、CD4+、CD8+细胞含量,CD4+/CD8+值。结果:(1)A、B、C组肿瘤体积较D组均有减少,提示与对照组相比,各治疗组均有不同程度的肿瘤抑制作用,而C组小鼠肿瘤生长受抑最为明显。(2)A、B、C组瘤重分别与D组比较,结果均有减少,其中C组减少最为明显。(3)B、C两组小鼠肿瘤组织经加热后,细胞浆内的热休克蛋白较未加热的A、D两组明显增加。(4)A、B、C组与D组相比较CD8(CTL)均有提高,而以C组t最为显著,提示联合治疗组对CTL增殖的刺激作用更加显著。结论:E1B-55KD基因缺陷腺病毒联合热疗对肿瘤的治疗有协同作用。
Objective： To investigate synergistic effect of gene therapy combined with thermotherapy. Methods： Fourty tumorbearing mice were randomly divided into 4 groups： A group （infected by E1 B-55 KD gene defect adenovirus）, B group （treated by thermotherapy） , C group （infected by E1B-55KD gene defect adenovirus and treated by thermotherapy） , D group （control）. The tumour＇s diameter and weight were mearsued before and after the experiment; the HSP were located and quantitated by Western blot; the T cell subgroup were detected by flow cytometry and the content of CD3^＋, CD4^＋ , CD8^＋, CD4^＋/CD8^＋ were calculated. Re. stilts： （1） Compared with D group, tumor in all therapy group were depressed, which in C group was restrained extremely. （2） Compared with D group, the weight of tumor was reduced in A, B, C group, among them C group reduced significantly. （3） By heating the tumor, the HSP of cytolymph was obviously increased in B, C group, but which not occurred in A, D group. （4） Compared with D group, CD8 （CTL）was elevated in A, B, C group; among them C group elevated significantly; all these reflect that therapeutic alliance had more promoting effect on the generation of CTL. Conclusion： E1B-55KD gene defect adenovirus combined with therrnotherapy has synergistic healing effect on tumor.
出处
《沈阳医学院学报》
2009年第3期148-150,共3页
Journal of Shenyang Medical College
