摘要
研究了不同温度(5～35℃)对雨生红球藻(Haematococcus pluvialis)叶绿素荧光特性及虾青素含量的影响。测定的主要参数有:PSII的最大光能转化效率(Fv/Fm)、PSII的潜在活性(Fv/Fo)、PSII的实际光能转化效率(ΦPSⅡ)、光合电子传递效率(ETR)、细胞密度、叶绿素相对含量以及虾青素含量。单因子方差分析结果表明:在整个培养周期中,温度对雨生红球藻各叶绿素荧光参数、叶绿素相对含量、细胞密度以及虾青素含量均有显著影响(P<0.05)。多重比较结果表明,在本实验条件下,雨生红球藻的最适生长温度是20℃,其单位体积虾青素含量以20℃处理组为最高,而单个细胞中的虾青素含量则以5℃和30℃为最高。相关性分析结果表明:培养天数为3～10天时,叶绿素相对含量与细胞密度均成显著正相关关系。本文还初步探讨了叶绿素荧光技术在筛选耐高温或耐低温微藻品系中的应用。
In this paper, the effects of different temperatures （5- 35℃） on the chlorophyll fluorescence parameters and astaxanthin content of Haernatococcus pluvialis were examined. The main parameters determined in the present study included the maximal photochemical efficiency of PSII （Fv/Fm）, the potential activity of PSⅡ （Fv/Fo）, the actual photochemical efficiency of PSⅡ in the light （φPS Ⅱ ）, the relative electron transport rate （ETR）, cell density, relative chlorophyll content, and astaxanthin content. One-way analysis of variance showed that temperature had significant effects on the fluorescence parameters, relative chlo
rophyll content, cell density and astaxanthin content of H. pluvialis during the entire experimental period （P〈0.05）. Multiple comparisons show that the optimal growth temperature of H. pluvialis under the experimental conditions was 20 ℃. The astaxanthin content of H. pluvialis expressed by ug/mL showed the highest valve at 20℃ while that expressed by ug/cell reached the highest value at 5℃ and 30℃. The analysis of correlations showed that there were significant positive correlations between relative chlorophyll content and cell density from day 3 to day 10. In addition, the possibility of using chlorophyll fluorescence induction kinetics in selecting high or low temperature resistance microalgal strains was also discussed in this paper.
出处
《海洋湖沼通报》
CSCD
北大核心
2009年第3期112-120,共9页
Transactions of Oceanology and Limnology
基金
"十一五"国家科技支撑计划(2006BAD09A03)资助
关键词
雨生红球藻
温度
叶绿素荧光
虾青素
haematococcus pluvialis
temperature
chlorophyll fluorescence parameters
astaxanthin content
