摘要
目的探讨尿蛋白电泳与尿白蛋白(Alb)/肌酐(Cr)比值诊断糖尿病(DM)早期肾损伤的临床价值。方法采用十二烷基磺酸钠—琼脂糖凝胶蛋白电泳技术对316例DM患者行尿蛋白电泳检测,免疫比浊法行尿Alb检测,碱性苦味酸法行尿Cr检测,并计算尿Alb/Cr比值。结果尿Alb/Cr比值异常176例,其尿液蛋白图谱中除有深而宽的Alb条带外,还出现大分子和(或)小分子蛋白条带;而其比值正常的140例中,54例除有Alb条带外,还有大分子和(或)小分子蛋白条带。结论尿Alb/Cr比值可作为评价DM早期肾损伤的一项实验室指标;其比值正常不能作为否定DM患者存在早期肾损伤的依据,应加行尿蛋白电泳检测,明确是否存在早期肾损害。
Objective To investigate the early diagnostic evaluation of urinary protein electrophoresis and urine albumin/creatinine ratio in diabetic renal injury. Methods Urine proteins of three hundred and sixteen diabetic mellitus patients were detected by sodium dodecylsulphate（SDS）-agarose gel protein electrophoresis（AGE）. Urine albumin level was detected by immunoturbidimetry, and using alkaline picric acid method for determination of urinary creatinine, and carry out random urinary albumin/creatinine ratio. Results In addition to the deep and wide electrophoresis strip of albumin, were the bands of other maeromolecular and/or small-molecular protein not only detected in 176 urinary samples from the diabetes patients with the abnormal urinary albumin/creatinine ratio, but also in 54 urine samples from the patients with normal urinary albumin/creatinine ratio. Conclusions The urinary albumin/creatinine ratio can be used for the evaluation of early diabetic renal injury in a laboratory, but the normal urinary albumin/creafinine ratio can not be used to deny the existence of a renal injury for the diabetic patients, and urine protein electrophoresis should be considered for further test to determine whether the early renal damage exist or not.
出处
《山东医药》
CAS
北大核心
2009年第37期5-6,共2页
Shandong Medical Journal
基金
江西省卫生厅科技计划项目(赣卫科教字20081104)
关键词
糖尿病肾病
尿蛋白电泳
尿白蛋白
肌酐
diabetic nephropathies
urine protein electrophoresis
injury of kidney
urinary albumin
creatinine
