摘要
目的根据超声破坏微泡造影剂可增加细胞膜通透性的原理，探讨一种新的受体介导基因转移技术。方法将c-myc基因反义寡核苷酸（ASODN）、荧光素标记的特异性配体半乳糖基化多聚赖氨酸（G-PLL）与载体SonoVue微泡结合，用超声介导微泡破裂的方法使cmycASODN转染于人肝癌细胞SMMC7721，观察其对癌细胞的作用。结果体外实验结果显示，1．5MHz脉冲波，10％工作周期，机械指数1．0W／cm^2、60S超声介导10％SonoVue微泡对细胞活性无明显影响；半定量逆转录聚合酶链式反应（RT-PCR）显示实验组（治疗基因c-mycASODN＋G-PLL＋微泡＋超声）c-mycmRNA基因表达水平显著降低（P〈0．01）。结论受体介导的靶向招声微泡措影剂存韶声作用下能有效地促进目的基因在肝痛细胞中的表达，为肝癌基因治疗提供了新的徐径。
Objective To develop a novel approach of transfecting the exogenous gene safely and effectively mediated by receptor according to the cavitation effeet of ultrasound-mediated mierobubble destruction. Methods C myc antisense oligonucleotide（ASODN） and galaetosylation polylysine（G-PLL） which was fluoresce in labeled specificity iigand and SonoVue microbbule were coupled by electrostatic interaetion. SMMC 7721 eell with c-mye ASODN were transfeeted in vitro by ultrasound-mediated mierobubble destruction to investigate the effect of c-myc ASODN on it. The e-myc mRNA gene expression of liver cancer SMMC-7721 cells was detected. Results Cultured with 10% SonoVue microbubble and under ultrasound at 1.5 MHz, 1.0 W/cm^2, 10% duty cyele,60 s respectively,the SMMC-7721 cells had not been changed significantly. Reverse transcription polymerase chain reaetion（RT-PCR） assay showed that c-myc expression was decreased in transfeeted eells（ P 〈0.01）. Conclusions Recepto-mediated targeted microbubble destruction by ultrasound can enhance the exogenous gene transfeetion and expression. It is a promising method in gene therapy of liver cancer.
出处
《中华超声影像学杂志》
CSCD
北大核心
2009年第9期809-812,共4页
Chinese Journal of Ultrasonography
基金
黑龙江省自然科学基金（D2004-14）
黑龙江省教育厅科学技术研究项目（10541129）
关键词
超声检查
微气泡
转染
细胞系
肿瘤
基因
MYC
Ultrasonography
Microbubbles
Transfection
Cell line, tumor
Genes, myc
