摘要
[目的]利用韧带先进加强系统(ligament advanced reinforcement system,LARS)重建实验动物的前交叉韧带(anterior cruciate ligament,ACL),研究LARS人工韧带与骨界面结合的情况。[方法]取雄性成熟波尔山羊24只,随机分成3组。利用临床ACL重建系统建立动物实验模型。分别于术后4、8、12周取材做大体观察、组织学、影像学和生物力学检查。所得实验数据进行统计分析。[结果](1)术后4周组织学显示韧带-骨界面充满大量的疏松结缔组织,可见到炎细胞浸润表现;术后8周韧带-骨界面之间有新骨形成。部分标本出现Sharpey纤维结构;术后12周韧带-骨界面见大量的Sharpey纤维结构及大量的成纤维细胞。未见钙化软骨;(2)对第3组实验动物术后4、8、12周进行影像学检查,然后利用eflime图像处理软件所得数据进行统计学分析。结果显示有显著性统计学差异(P<0.01);(3)生物力学测试发现,在不同时间点均将韧带从骨隧道中拉出,韧带无一断裂。结果显示没有统计学差异(P>0.05)。[结论]LARS人工韧带重建ACL后,其两端骨隧道中韧带-骨界面可通过Sharpey纤维结构,形成间接连接促进韧带骨的结合增强韧带骨愈合的强度。
[ Objective ] To reconstruct anterior cruciate ligament （ACL） by using ligament advanced reinforcement system （LARS） , and to investigate the integration of LARS artificial ligament with bone interface in the animals with regard to imageology, biomechanics and histology. [ Method ] Twenty-four Boer goats were randomly divided into three groups. The animal mode of ACL reconstruction was established by clinical ACL reconstruction system. Gross observation was made, and histological, ima- geological and biomechanical changes were observed at 4, 8 and 12 weeks after surgery, respectively. Statistical analysis was per- formed. [ Result] （1）At 4 weeks after surgery,ligament-bone interface had great amount of loose bindweb and infiltration of chro- nic inflammation cells. At 8 weeks after surgery, there was new bone formation. Part of samples had Sharpey fibers. At 12 weeks after surgery, Sharpcy fibers, and a large number of fibroblasts were noted in the interface between LARS artificial ligament and bone interface. But calcified cartilage was not founded. （2）The imageology examination for group 3 was made at 4, 8 and 12 weeks after surgery. The data were analyzed statistically by the image processing software of eflime, and there was evident statisti- cal difference （P 〈 0.01 ） . （3）The biomechanics examination showed that the ligaments were pulled out from the bone tunnel, and no ligament was ruptured. There was no statistical difference （ P 〉 0.05 ） . [ Conclusion] After ACL reconstruction by LARS artificial ligament, indirect connection developed via Sharpey fibers in bone tunnel at the end of LARS artificial ligament and bone interface. The integration of LARS artificial ligament with bone interface has been improved and its intensity is increased.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2009年第18期1407-1410,共4页
Orthopedic Journal of China
关键词
LARS
人工韧带
骨界面
结合
LARS
artificial ligament
bone interface
integration
