摘要
目的研究罗格列酮在不同浓度葡萄糖条件下对B细胞株NIT-1细胞增殖、凋亡及胰岛素分泌的影响。方法将NIT-1细胞按5×104个细胞/孔至24孔培养板中培养,根据培养基RPM1640葡萄糖浓度不同分为以下5组:G1组(5.6 mmol/L)、G2组(11.1 mmol/L)、G3组(16.7 mmol/L)、G4组(22.2 mmol/L)及G5组(27.6 mmol/L),继续培养72 h后,分别给予10-5mmol/L罗格列酮干预48 h后,取上清液采用放免法检测各组胰岛素水平;采用免疫荧光法检测凋亡细胞,MTT法检测细胞增殖情况。结果(1)罗格列酮能够明显抑制高浓度葡萄糖诱导的NIT-1细胞凋亡。施加罗格列酮浓度10-5mmol/L 48 h时后,能够明显抑制16.7 mmol/L、22.5 mmol/L和27.6 mmol/L浓度的葡萄糖所诱导的细胞凋亡。(2)罗格列酮能显著增加各浓度葡萄糖组NIT-1细胞的增殖活性。(3)罗格列酮能显著增加各葡萄糖浓度组的NIT-1细胞胰岛素分泌。结论罗格列酮可以通过直接作用于胰岛B细胞调节改善的胰岛B细胞的增殖及功能、抑制凋亡。
Objective To explore the roles of rosiglitazone in islet B cells lines （NIT-1 cells） proliferation,apoptosis and secretion induced by high concentration glucose in vitro. Methods The NIT-1 cells were cultured with different concentrations of glucose in RPM1640 in 24 well plates at a density of 5 × 104, and divided into the following groups：G1, G2, G3, G4 and GS, with glucose of 5.6,11.1,16.7,22.5 and 27.6 mmol/L,respectively. After culture for 72 hours, all the cells were treated with 10^-5 mmol/L rosiglitazone for 48 hours, then cells insulin level was evaluated by radioimmunoassay ,cells apoptosis was examined by Hoechst 33342 assay ,and cells proliferation was detected by MTT. Results （ 1 ） Rosiglitazone was able to inhibit NIT-lcells apoptosis caused by high concentration of glucose, after 48 hours＇ treatment with rosiglitazone （ 10^-5 mmol/L） ,cells apoptosis caused by glucose with concentration of 16.7 mmol/L,22.5 mmol/L and 27.6 mmot/L was significantly inhibited. （2） Rosiglitazone was able to enhance NIT^-1 cells proliferation. （3） Rosiglitazone was able to improve NIT^-1 cells insulin secretion in different glucose concentration. Conclusion Rosiglitazone can improve the islet B cells proliferation and function as well as inhibit apoptosis through direct effect on the islet B cells.
出处
《广西医学》
CAS
2009年第9期1231-1233,共3页
Guangxi Medical Journal
基金
国家自然科学基金(30860116)
广西科学基金(桂科青0339026)
广西科学基金(桂科自0542083)
