摘要
目的探讨O3和NO2单独和混合暴露对小鼠气道的损伤和炎症水平影响。方法将80只清洁级BALB/C小鼠随机分为8组:其中4组为致敏模型组(根据暴露不同分为空气对照组、O3组、NO2组及混合暴露组),另外4组为非致敏组(分组同前)。O3暴露浓度采用0.16mg/m3,NO2为0.30mg/m3,每天暴露2h,连续7d。暴露完成后处死动物进行气管灌洗,取支气管肺泡灌洗液(BALF)进行细胞计数和细胞因子含量检测。结果致敏组中,NO2和O3单独和混合暴露后均可导致BALF中性粒细胞比例、IL-6水平明显升高(均为P<0.05),其中O3单独暴露和混合暴露还导致IL-2和MDA的明显升高(均为P<0.05);非致敏组中,只有O3暴露和混合暴露可引起BALF细胞总数、中性粒细胞比例增高和IL-2、IL-6、MDA水平明显升高(均为P<0.05),且致敏组IL-2和IL-6水平高于相同暴露条件非致敏组(P<0.05);混合暴露时致敏小鼠气道内细胞总数和MDA明显高于O3或NO2单独暴露(P<0.05)。结论O3和NO2暴露均会加重致敏小鼠气道炎症,其中O3暴露还会引起非致敏小鼠气道炎症发生,引起炎症反应均是以中性粒细胞增高为主要特征;混合暴露对小鼠气道炎症存在联合作用;相对于非致敏小鼠,致敏小鼠对O3和NO2暴露更敏感。
Objectlve To investigate the effects of single and combined exposure to ozone and nitrogen dioxide on airway injury and inflammatory levels in mice. Methods Eighty BALB/C mice were randomly divided into eight groups： four were sensitized model groups （the control group, the O3 group, the NO2 group and the O3＋NO2 group based on the way of exposure）, the other four were non-sensitized groups. The concentration of O3 was 0.16 mg/m3, NO2 was 0.30 mg/m3. Mice were exposed 2 hours every day for 7 consecutive days. Mice were sacrificed in the exposure end and cell counting and cytokine measurement from bronchoalveolar lavage fluid （BALF） were performed. Results In the sensitized groups, neutrophil proportion and IL-6 were significantly increased except the control group and IL-2 and MDA were also significantly increased in the O3 group and the O3＋NO2 group （P〈0.05）; In non-sensitized groups, total cell count, neutrophil proportion,IL-2,IL-6 and MDA were significantly increased only in the O3 group and the O3＋NO2 group （P〈0.05）,and IL-2 and IL-6 in sensitized groups were higher compared with non- sensitized groups （P〈0.05）; Total cell count and MDA of combined exposure were significantly higher than single exposure in sensitized groups （P〈0.05）. Conclusion NO2 and O3 may aggravate airway inflammation in sensitized mice, moreover O3 may induce airway inflammation in non-sensitized mice and the inflammation is featured as neutrophilic granulocytosis; O3 and NO2 have combined effect in airway inflammation in mice, and the sensitized mice are more sensitive than non-sensitized mice.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2009年第9期783-786,共4页
Journal of Environment and Health
基金
国家科学技术部"十一五"支撑计划项目(2006BAI19B06)
关键词
臭氧
二氧化氮
气道炎症
联合作用
Ozone
Nitrogen dioxide
Airway inflammation
Combined effect
