摘要
目的:探究放、化复合损伤性血虚证小鼠造血调节因子及其受体水平改变,丰富血虚证实质研究内涵。方法:用荧光定量PCR检测放、化复合损伤性血虚证小鼠肾脏Epo、脾脏Epo受体、骨髓细胞GM-CSF mRNA表达量的变化。结果:放、化复合损伤性血虚证小鼠肾脏Epo、脾脏Epo受体mRNA表达量显著高于正常小鼠,骨髓GM-CSF mRNA表达量无显著改变。结论:放、化复合损伤性小鼠血虚模型的形成机制与肾脏Epo、脾脏Epo受体和骨髓细胞GM-CSF的减少无关。
Objective： To explore the changing of hematopoietic regulation factors and their receptors levels in radiation, chemistry injury blood deficiency syndrome mice, enrich the material basis researches of blood deficiency syndrome. Methods： Using fluorescent real-time quantitation PCR to observe the changes of the kidney Epo, the spleen EpoR and the bone marrow cells GM-CSF mRNA quantities in radiation, chemistry injury blood deficiency syndrome mice. Results： The expression quantities of the kidney Epo, the spleen EpoR mRNA arc obviously higher in radiation, chemistry injury mice than normal mice, the bone marrow cells GM-CSF mRNA quantity are not obviously different between two groups. Conclusions： The forming mechanisms of blood deficiency in radiation, chemistry injury mice do not related with the reduces of kidney Epo, the spleen El）oR and the bone marrow ceils GM-CSF.
出处
《成都中医药大学学报》
2009年第3期56-58,共3页
Journal of Chengdu University of Traditional Chinese Medicine
基金
四川省卫生厅科研项目(编号:060095)
关键词
放、化复合损伤
血虚证
动物模型
造血调节因子
基因表达
荧光定量PCR
radiation, chemistry injury
blood deficiency syndrome
animal mode
hematopoietic regulation factors
gene expression
fluorescent real-time quantitation PCR
