摘要
对用双水相分配技术结合温度诱导相分离技术从酵母中提取谷胱甘肽（GSH）进行了研究．考察了GSH在环氧乙烷──环氧丙烷无规共聚物（EOPO）／羟丙基淀粉（PES）系统中的分配行为，包括两次分离过程（双水相分配及温度诱导过程)中的不同系统组成、pH等对GSH分配的影响，确定了较佳的双水相系统──EOPO400013％，PES10010％，pH=10.5．在此基础上进行了从酵母细胞中提取GSH的工艺研究，设计了合理的分离流程，研究结果表明GSH的总萃取率可达80％以上．
The techniques for separation of glutathione（GSH）by aqueous two-phase extraction combined with temperature-induced phase separation were studied.The partition behavior of GSH in ethylene oxide-propylene oxide random copolymer(EOPO）/hydroxypropyl starch（PES)／water systems was investigated．The effects of such factors,as concentration of polymer,pH,on the partition coefficients in two separation processes（aqueous two-phase extraction and temperature-induced phase separation)were studied.The optimum conditions were EOPO4000 13%,PES100 10％,pH=10.5. The separation technique for GSH from yeast cell was also investigated.A feasible separation scheme was suggested,and the results show that the total extraction yield could reach over 80％．
出处
《化工学报》
EI
CAS
CSCD
北大核心
1998年第4期470-475,共6页
CIESC Journal
基金
国家自然科学基金!29731680
29706009
关键词
温度诱导相
分离
谷胱甘肽
提取
酵母细胞
aqueous two-phase system,temperature-induced phase separation,glutathione,ethylene oxide-propylene oxide random copolymer
