摘要
目的:探讨MR弥散加权成像(DWI)评价大鼠肝纤维化的能力。方法:采用四氯化碳(CCl4)腹腔注射的方法建立22只SD大鼠肝纤维化的动物模型,每周随机取2～4只行DWI,测定其表观弥散系数(ADC)。将动物处死后取肝脏进行病理学胶原染色,同时依据Ishak评分标准对肝纤维化程度进行评分。根据Ishak评分结果将动物分组,计算每组ADC值与病理评分间的相关性。结果:在肝纤维化早期,b=500的ADC值与Ishak评分呈负相关(相关系数分别为r=-0.914,P<0.001)。但肝纤维化中晚期,各b值的ADC值与Ishak评分间不具相关性。结论:DWI的ADC值在肝纤维化早期能定量反映纤维化的程度,在中晚期肝纤维化时不能反映纤维化的病变程度。
Objective To investigate the ability of diffusion-weighted MR imaging （MR-DWI） to detect and quantify the degree of liver fibrosis in SD rats. Methods Model of liver fibrosis was established by intraperitoneal injection of carbon tetrachloride （CC14） in 22 SD rats. MR-DWI was performed in 2-4 randomly selected rats every week after the injection of CCL4, and the fibrosis liver taken from the rat immediately after MR scanning was studied pathologically using Sirius red stain. The histological grade of fibrosis was scored according to Ishak scoring criteria. Animals were grouped according to Ishak score. Relationship between apparent diffusion coefficient （ADC） value and Ishak score was analyzed by Spearman process. Results At the early stage of liver fibrosis, when b value = 500 the apparent diffusion coefficient （ADC） value was negatively correlated with Ishak score （relationship coefficients r=-0.914, P〈0.001）, whereas there was no relationship between ADC value and Ishak score in late liver fibrosis and cirrhosis. Conclusions ADC value of MR-DWI can be used as an indicator to reflect the severity of liver fibrosis at early stage, whereas liver fibrosis at advanced stage could not be quantified by DWI.
出处
《诊断学理论与实践》
2009年第4期405-409,共5页
Journal of Diagnostics Concepts & Practice
基金
上海市重点学科建设项目资助(S30203)
关键词
肝纤维化
肝硬化
磁共振成像
弥散加权成像
动物
实验
Liver fibrosis
Liver cirrhosis
Magnetic resonance imaging
Diffusion-weighted imaging
Animal, experimental study
