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用于分枝杆菌分子生物学鉴别诊断的靶序列 被引量：3

Target sequences used in molecular biological diagnosis of Mycobacterium infection
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摘要 分子生物学技术已应用于分枝杆菌感染的辅助诊断。目前分子鉴定靶序列主要有4种：即hsp65基因、dnaJ基因、16SrRNA基因和16S-23SrRNA间隔区序列。其他一些序列如IS6110、间隔子寡核苷酸、rpoB基因和mRNA，属于种特异性或种群特异性DNA序列，可用于结核分枝杆菌的鉴别诊断和耐药性检测。本文对这些DNA序列在分枝杆菌鉴定中的应用作一综述。 Molecular biology technology has been used in diagnosis of Mycobacterium infection.Hsp65 gene, dnaJ gene, 16SrRNA and 168-23SrRNA are widely used as target sequences. 186110 gene, spacer oligonueleotides, rpoB gene and mRNA are genus specific or species specific sequences, which can be used in Mycobacterium tuberculosis differential diagnosis and detection of drug resistance. The paper summarise the DNA sequences used for discrimination of Mycobacterium.
作者 张峰 李子玲 施毅
机构地区 第二军医大学南京临床医学院南京军区南京总医院呼吸内科
出处 《国际呼吸杂志》 2009年第17期1068-1071,共4页 International Journal of Respiration
关键词 分枝杆菌 分子生物学 鉴别诊断 Mycobacterium Molecular biology Differential diagnosis
分类号 R450 [医药卫生—治疗学]
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参考文献20

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国际呼吸杂志
2009年 第17期

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