摘要
目的研究准确定量检测ＩｇＭ、ＩｇＧ、ＩｇＡ型类风湿因子（ＲＦ）的方法。方法应用生物素－亲和素结合ＥＬＩＳＡ法（ＡＢＣ－ＥＬＩＳＡ）对５７例类风湿关节炎患者及１００例正常人血清进行检测，并与胶乳凝集试验（ＬＡＴ）及常规ＥＬＩＳＡ法测定的ＲＦ作对比。结果５７例病人ＩｇＭＲＦ阳性率在ＡＢＣ、ＥＬＩＳＡ法及ＬＡＴ法分别为８９．５％，８０．７％和６３．２％（Ｐ＜０．０１）。在ＬＡＴ法ＩｇＭＲＦ阴性的２１例中，ＡＢＣ－ＥＬＩＳＡ法能测出１５例阳性，此外５７例病人共测出ＩｇＧＲＦ２０例阳性，ＩｇＡＲＦ２９例阳性。结论ＡＢＣ－ＥＬＩＳＡ法能定量检测ＲＦ，并能明显提高常规ＥＬＩＳＡ法的敏感性，有较高的实用价值。
Objective\ To study the method for accurate quantitation of IgM RF,IgG RF and IgA RF.\ Methods \ The biotinavidin system combined ELISA assay(ABCELISA) was applied for the detection of IgM rheumatoid factor(RF),IgG RF and IgA RF in sera of 57 patients with rheumatoid arthritis and 100 healthy controls,and compared with standard ELISA and latex agglutination tests(LAT).\ Results\ In the former group,the ABCELISA,standard ELISA and LAT had their positive rates of IgM RF of 89.5%,80 7% and 63 2%,respectively(P<0 01).\ IgM RF with LAT negative result was positive by ABCELISA in 15 cases.\ In addition,20 sera IgG RF and 29 sera IgA RF were positive in 57 patients by ABCELISA assay.\ Conclusion\ The ABCELISA assay for RF is more sensitive than standard ELISA or LAT.\ This quantitative RFs detecting technique may have much practical value.
出处
《福建医科大学学报》
1998年第1期53-55,共3页
Journal of Fujian Medical University
基金
福建省教委三项费资助
