摘要
目的观察增殖细胞核抗原（ＰＣＮＡ）基因反义ＲＮＡ表达质粒对人胃癌细胞生长特性的影响，探讨其抗肿瘤作用。方法用ＤＮＡ重组方法将人ＰＣＮＡ基因反向克隆到真核表达质粒ｐＤＯＲｎｅｏ中，构建成人ＰＣＮＡ基因真核表达质粒ｐＤＲＰＣＮＡ，用脂质体介导转染人胃癌细胞系ＳＧＣ７９０１。结果经Ｇ４１８筛选获得转染细胞ＳＧＣ／ＰＣＮＡ，与亲本细胞相比，其生长速度减慢，ＲＮＡ、蛋白质的生物合成受到抑制，Ｓ期、Ｇ２／Ｍ期ＤＮＡ含量降低。结论ＰＣＮＡ基因反义ＲＮＡ对胃癌细胞的生长具有明显的抑制作用。
Objective To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell. Methods An eukaryotic expression vector pDR PCNA with human PCNA gene insert in reverse direction was successfully constructed. The human gastric cancer cell line SGC 7901 was transfected with pDR PCNA by lipofectamine TM . Results Growth rate, protein and RNA biosynthesis and DNA contents in S phase and G 2/M phase of the transfected SGC 7901 were significantly suppressed in comparison with those of the parental cell line. Conclusion PCNA antisense RNA can significantly suppress the in vitro growth of gastric cancer cell line.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1998年第5期337-339,共3页
Chinese Journal of Oncology
关键词
胃肿瘤
细胞株
增殖细胞核抗原
基因治疗
Stomach neoplasms
Cell line
Proliferating cell nuclear antigen
RNA
antisense
Gene therapy
