摘要
目的:寻找一种冰冻切片间接免疫荧光染色以及HE染色过程中最佳的组织固定方法。方法:取健康成年小鼠双侧睾丸冰冻切片,分别采用3种方法进行固定:①丙酮4℃固定15分钟,-20℃保存24h;②95%酒精4℃固定15分钟,-20℃保存24h;③切片-20℃保存24h后,再用4℃丙酮固定15分钟。然后做间接免疫荧光和HE染色,观察组织结构及染色情况。结果:4℃丙酮固定的组织切片HE染色以及间接免疫荧光效果良好。-20℃保存24h后,4℃丙酮固定15分钟的冰冻切片HE染色和间接免疫荧光结果最差。结论:酒精和丙酮都可以固定组织,但是4℃丙酮固定效果最好。
Objective： To find a best fixation metbod for indirect immunofluorescence and HE staining of frozen section. Methods： Segregate testis from healthy adult mouse ,were fixed at 4℃ acetone for 15min ,or fixed at 4℃ 95% alcohol for 15min, or fixed at 4℃ acetone for 15 min after they were saved at -20℃ for 24h. Then each sample was used for indirect immunofluorescence and HE staining. Results：Indirect immunofluorescence and HE staining were showed that the better result and fine structure were obtained when the frozen section was fixed at 4℃ acetone for 15min. After they were saved at -20℃ for 24h and then fixed at 4℃ acetone for 15min, results were poor. Conclusion：acetone and alcohol could all be used to fix testis while apparently acetone fixed at 4℃ lives the best than others.
出处
《农垦医学》
2009年第4期298-300,共3页
Journal of Nongken Medicine
关键词
冰冻切片
组织固定
间接免疫荧光
HE染色
Frozen sections
Tissue fixation
indirect immunofluorescence
HE staining
