摘要
目的探讨两种植物雌激素（染料木素、大豆甙元）对子宫内膜癌细胞株（Ishkawa细胞系）中雌激素受体相关受体α（estrogen-related receptorα,ERRα）的表达及细胞增殖的影响。方法采用Real-Time PCR方法检测不同浓度（40、20、10、5μmol/L）的两种植物雌激素作用于Ishkawa细胞系不同时间（24、48 h）后ERRαmRNA的变化,并用MTT法检测细胞的增殖情况。结果Ishkawa细胞系经低浓度染料木素处理后ERRαmRNA的表达均有上调趋势,以10-20μmol/L较为明显,40μmol/L浓度时ERRαmRNA的上调受到抑制。且染料木素在低浓度（5、10μmol/L）对Ishkawa细胞的增值有着抑制作用,而在较高浓度（20、40μmol/L）有着促进Ishkawa细胞生长的作用。大豆甙元处理24 h组ERRαmRNA均有上调,以40μmol/L最为明显;48 h组均有上调,以20μmol/L上调最为明显,40μmol/L浓度时ERRαmRNA的上调受到抑制。且24 h和48 h组各浓度均对Ishkawa细胞增殖有着抑制作用,24 h以低浓度抑制较为明显,48 h组各浓度之间增值率的差异不明显。结论大豆甙元和染料木素在低浓度时均可上调ERRdmRNA并抑制Ishkawa细胞增殖，在高浓度时却对Ishkawa细胞ERRetmRNA的上调作用不明显，染料木素高浓度时促进Ishkawa细胞增殖，大豆甙元高浓度时抑制Ishkawa细胞增殖作用较低浓度为差。
Objective To study the influence of two phytoestrogens（Genistein and Daidzein） on estrogen-related receptor-α in endometrial cancer cell line Ishkawa,and on the proliferation of the cells in this cell line.Methods Ishkawa cells were incubated with different concentrations of Genistein and Daidzein（40,20,10,5 μmol/L） for 24 h or 48 h,followed by Real-Time PCR for analyzing the expression of ERR-α mRNA in the cell line.MTT assay was then performed to evaluate the proliferation of Ishkawa cells.Results The expression level of ERR-α mRNA in Ishkawa cells was higher than that of the control group after being dealt for 24 h or 48 h with Genistein, and the concentration 20 μmol/L was most effective. Nevertheless, this up-regulation was blocked when the cells were treated with 40 μmol/L Genistein. Lower concentration （5,10 μmol/L） Genistein had depressant effect on proliferation of the cells, while higher concentrations （20, 40 μmol/L） had stimulant effect. After being treated with different concentrations of Daidzein, the expression of ERR-α mRNA in all experimental groups was significantly higher than that in the control group. In the 24 h group, the concentration 40 μmol/L had most obvious effect; but in the 48 h group, the concentration 20 μmol/L had most obvious effect, and this up-regulation was blocked when the concentration was elevated to 40 μmol/L. Noticeably, all concentrations of Daidzein had depressant effect on the proliferation of Ishkawa cells in both 24 h and 48 h groups. In the 24 h group, lower concentrations were more effective, but in the 48 h group, concentration showed no significant effect. Conclusion In lower concentrations, both Genistein and Daidzein have up-regulation effect on the expression of ERR-α, and block the proliferation of Ishkawa cells; but in higher concentrations, the up-regulation effect on ERR-α mRNA expression by these two phytoestrogens is not obvious. Genistein stimulates the proliferation of Ishkawa cells in higher concentrations, while Daidzei
出处
《同济大学学报（医学版）》
CAS
2009年第4期12-17,共6页
Journal of Tongji University(Medical Science)
