摘要
目的研究羊肚菌多糖提取的最佳方法,并比较不同种羊肚菌在多糖得率上的区别。方法以羊肚菌GIM5.69[(Morchella esculenta(L.)Pers.)]及黑脉羊肚菌GIM5.29(Morchella elata)的菌丝体作为实验对象,研究与常规热水浸提方法相比,超声及酶处理对多糖得率的不同影响,并比较两类羊肚菌在多糖得率上的区别。结果超声和酶法均可显著提高多糖得率,其中以超声10min提高效果最明显。不同类型的菌种在多糖产量上存在明显差异,GIM5.69型菌种多糖得率明显高于GIM5.29型。结论羊肚菌多糖最佳提取分离条件为:超声10min,超声频率为40kHz,在90℃下恒温加热150min,浸提2次。
Objective To optimize the extraction technology of Morchella polysaccharide. Methods With GIM5.69 （Morchella esculenta （L.） Pers.） and GIM5.29 （Morchella elata） as the research object, the difference of polysaccharide yield with lixiviation in polysaccharide extraction between ultrasound and enzyme was observed. At the same time, the difference of polysaccharides yield of these two Morchella was compared. Result Ultrasound and enzyme both can improve the polysaccharides yield, and the effect of 10 rain of ultrasound was the best. The polysaccharides yield of GIM5.69 was higher than that of GIM5.29, which means different strain has different polysaccharides yield. Conclusion The best extraction condition is 10 min of ultrasound at the frequency of 40 kHz, heating 150 min at 90 ℃ and lixiviation twice.
出处
《中国中医药信息杂志》
CAS
CSCD
2009年第9期40-42,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
2009年高等学校安徽省自然科学研究项目(KJ2009A172)
关键词
羊肚菌
黑脉羊肚菌
多糖
提取工艺
Morchella esculenta
Morchella elata
polysaccharides
extraction technology
