期刊文献+

检测禽流感病毒H5、H7和H9亚型的正向基因芯片的制备 被引量:5

Preparation of Microarrays For Detection of Aiv Subtypes H5,H7 And H9
下载PDF
导出
摘要 通过分析比较禽流感病毒H5、H7和H9亚型不同毒株的基因组序列,设计出H5、H7和H9亚型特异性引物与探针,将扩增产物点制在芯片上,分别用H5、H7和H9亚型特异性探针与芯片杂交,研制出用于禽流感病毒H5、H7和H9亚型检测的正向杂交基因芯片,同时对正向基因芯片进行特异性、敏感性试验,并用该方法对待检样品进行了检测。结果表明,禽流感病毒正向基因芯片与新城疫病毒、传支病毒等6种禽类常见病毒无反应,其敏感性高于常规PCR方法。 Primers and probes were designed specific for Avian influenza viruses subtypes H5、H7 and H9 based on the genomic sequences that published at GenBank database.Amplified products of H5,H7 and H9 by PCR or RT-PCR were fixed on the slide,and hybridized with specific probes for subtypes H5,H7 and H9 separately.A microarray for detecting avian influenza viruses subtypes H5,H7 and H9 were developed.Then its specificity and sensitivity were validated by standard strains and samples from different areas.The results showed that the method based on microarray was of high specificity and sensitivity, and the method can meet the demands of field detection of AIV subtypes HS, H7 and H9 contamination.
出处 《检验检疫科学》 2009年第4期12-16,共5页 Inspection and Quarantine Science
基金 国家科技攻关计划项目:禽流感病毒分型基因芯片检测技术研究(2004BA519A56-05)
关键词 禽流感病毒 基因芯片 检测 Avian influenza virus(AIV) Microarray Detection
  • 相关文献

参考文献6

二级参考文献50

  • 1杨素,花群义,徐自忠,杨晶焰,谭德勇,董俊,杨云庆,赖建华.口蹄疫等5种动物病毒基因芯片检测技术的研究[J].微生物学报,2004,44(4):479-482. 被引量:29
  • 2张应国,宋建领,胡媛媛,王金萍,张富强.禽流感病毒RT-PCR及多重RT-PCR检测技术的建立[J].中国兽医科技,2005,35(8):600-604. 被引量:34
  • 3徐翠玲,向妮娟,张烨,温乐英,赵翔,李梓,郭俊峰,王敏,余宏杰,杨维中,郭元吉,舒跃龙.2004—2005年中国A(H1N亚型流感病毒抗原性及基因特性研究[J].中华实验和临床病毒学杂志,2006,20(2):27-29. 被引量:27
  • 4陈红军,侯义宏,白华,陈桂来,吴时友,尹燕博,钱永华,田振宇.猪流感病毒分型基因芯片的建立和初步应用[J].畜牧兽医学报,2007,38(7):708-712. 被引量:15
  • 5Office International des Epizooties. List A and B Dise- ases of Mammals, Birds and Bees: Manual of Stan- dards for Diagnostic Tests and Vaccines [M]. 5th Edition. Paris:Office International des Epizooties,2004. 被引量:1
  • 6Starick E, Romer O A, Werner O. Type- and subtype-specific RT-PCR assays for avian influenza A viruses (AIV) [J]. J Vet Med,2000,47(4):295-301. 被引量:1
  • 7Munch M, Nielsen L P, Handberg K J, et al. Detection and subtyping (H5 and H7) of avian type A inf- luenza virus by reverse transcription-PCR and PCR-ELISA [J]. Arch Virol,2001,146(1):87-97. 被引量:1
  • 8Rona A M F, Theom B, Sander H, et al. Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene [J]. J Clin Microbiol,2000,38(11):4096-4101. 被引量:1
  • 9Ellis J S, Fleming D M, Zambon M C. Multiplex reverse transcription-PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996 [J]. J Clin Microbiol,1997, 35(8): 2076-2082. 被引量:1
  • 10Lee M S, Chang P C, Shien J H, et al. Identification and subtyping of avian influenza viruses by reverse transcription-PCR [J]. J Virol Methods,2001, 97: 13-22. 被引量:1

共引文献74

同被引文献94

引证文献5

二级引证文献32

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部