摘要
采用反相高效液相色谱法（RP-HPLC）测定了水产品中的三聚氰胺。色谱柱为SymmetryC18（5um，4．6mm×250mm），流动相为含有0．01mol／L庚烷磺酸钠和0．01mol／L柠檬酸的乙腈（1＋4）水溶液，检测器为紫外检测器，检测波长240nm。线性范围为1～50ug／mL，相关系数为0．9998，最低检出限为0．24ug／mL，样品平均加标回收率为78．8％～96．1％，相对标准偏差为3．7％～6．4％。方法可用于水产品中三聚氰胺的测定。
A method for determining melamine in aquatic product by RP-HPLC was described. The dctermination was carried out on a symmetry C18 reversed-phase column （5 um, 4.6 mm × 250 mm） at room temperature. A 20% （ V/V） acetonitrile-water mobile phase containing 0.01 mol/L 1-heptanesulfonic acid sodium salt and 0.01 mol/L citric acid with the flow rate at 1.0 mL/min was adopted and the detection wavelength was set at 240 nm. The calibration curve was linear in the concentration range of 1 - 50 ug/mL. The recoveries of 78.8 % - 96.1% by adding standard into samples were obtained, and the RSDs obtained for the within-run precision were 3.7 % - 6.4%. This method is simple, specific and accurate for monitoring concentration of melamine in aquatic product.
出处
《分析试验室》
CAS
CSCD
北大核心
2009年第9期116-118,共3页
Chinese Journal of Analysis Laboratory
基金
黑龙江水产研究所基本科研业务费专项(2007HSYZX-ZH-25)项目资助
关键词
三聚氰胺
水产品
反相高效液相色谱法
Melamine
Aquatic product
Reversed-phase high performance liquid chromatography
