摘要
采用H2O2氧化法降解壳聚糖制备的低聚壳聚糖诱导大叶黄杨植株并接种大叶黄杨白粉病菌，利用染色法测定处理后1～6d的大叶黄杨植株细胞壁中木质素、胼胝质和酚类物质的积累量变化，发现低聚壳聚糖诱导处理、接种大叶黄杨白粉病菌后诱导处理和诱导后接种处理都能提高大叶黄杨植株细胞壁中木质素、胼胝质和酚类物质的积累，但诱导后接种处理的大叶黄杨植株细胞壁中3种物质增加的速度最快，积累量最多，持续时间最长，诱导后第6d染色仍较重。这表明低聚糖可提高细胞壁的机械强度，从而增强了寄主的抗病性。
Oligochitosan prepared by the oxidation with H2O2 was used to induce Buxus megistophylla which was then inoculated by Powdery mildews. The accumulatative changes of lignin, callose and phenolics in B. megistophylla were measured by staining method during 1-6 d after inoculation. The results showed that the contents of lignin, callose and phenolics in cell wall of B. megistophylla plants, which were induced by oligochitosan, or inoculated with P. mildews after inducing, or induced after inoculating. However the treatment of inducing after inoculating presented the fastest speed, and large amount, and long duration accumulation, indicting that oligochitosan could increase mechanical strength of cell-wall to enhance the resistance of host.
出处
《西北林学院学报》
CSCD
北大核心
2009年第5期106-109,共4页
Journal of Northwest Forestry University
基金
国家"十五"科技攻关项目(2001BA501A09)
商洛学院科研基金项目(07KSY026)
关键词
低聚壳聚糖
大叶黄杨
白粉病
诱导抗病性
组织病理学
chitosan-oligosaccharide
Buxus megistoph ylla
Powdery mildews
induced disease-resistance histopathology
