摘要
利用微悬浮聚合技术,以二乙烯基苯、醋酸乙烯酯为单体,制备了微米级的顺磁性的聚合物微球,磁含量高于24%,饱和磁强度为16.7emu/g,平均粒径介于0.6~20μm,并在其表面修饰二乙烯基砜和2-巯基苯并噻唑。所制备的磁性聚合物微球,在低盐浓度下,依靠配基对抗体的电子-供电子（EDA）效应,可以实现磁性微球对人血清中的免疫球蛋白G（IgG）的特异性吸附行为。在解吸附过程中,洗提液中的盐种类对分离过程产生重要影响。温度对其吸附行为的影响较小,整个分离过程可以在室温条件下进行。利用这种嗜硫性磁球分离技术,条件温和,效率较高,分离出的抗体纯度较高,且生物活性没有损失。
A rapid and effective technique to specifically isolate immunoglobulin G （IgG） from human serum is developed based on the micro-sized thiophilic paramagnetic polymer beads. These magnetic beads were synthesized with vinyl acetate and divinylbenzene by microsuspension polymerization in the presence of the modified magnetite nanoparficles. Then, the thiophilic ligands of divinylsulphone and 2-mercapto-benzothiazole were grafted on the surface of these magnetic beads through Michael addition reaction. Depending on the cooperative action of divinylsulphone and 2-mercapto-benzothiazole, these magnetic particles exhibit a strong specificity towards IgG. Furthermore, this specific binding of IgG on the magnetic beads is performed at low salt concentration. It is much favorable to preserve the bioactivity of the antibodies and to increase the purity of IgG. However, the responding desorption is heavily depended on the types of salt in elution. Because the selective recognition between the thiophilic ligands and IgG is attributed to electron donor-acceptor （EDA） interaction, there is no obvious effect of temperature on the adsorption. Therefore, this isolation could be fully carried out at room temperature. Based on the magnetic separation technique and the appropriate magnetic particles, the isolation procedure was so rapid that 1.5 hours was enough to extract 72% IgG （98mg） from 10mL human serum.
出处
《材料科学与工程学报》
CAS
CSCD
北大核心
2009年第4期538-541,547,共5页
Journal of Materials Science and Engineering
基金
国家自然科学基金资助项目(50603007)
福建省高等学校新世纪优秀人才支持计划资助项目(07FJRC02)
关键词
顺磁性
微悬浮聚合
嗜硫性
免疫球蛋白G
分离
Superparamagneti
thiophilic
microsuspension
immunoglobulin
isolation
