摘要
目的探讨680nm激光对豚鼠图形视觉诱发电位(pattern visual evoked potential,PVEP)的影响。方法20只杂色豚鼠被随机分成对照组和实验组(680nm激光作用,北方电子设备研究所研制),每组10只。麻醉后,在前囟(breg-ma)前6mm、后10mm及矢状缝正中处钻孔,均植入长5mm、直径1.2mm的不锈钢螺钉作为参考电极、记录电极和地极。3d后,将清醒状态下的豚鼠固定于自制多功能头身一体化固定台面上,采用RETI-port系统(Roland Consult,德国)分别记录对照组和实验组图形视觉诱发电位的变化。结果在正常条件下可以记录到豚鼠稳定的PVEP图形,呈NPN形。激光刺激对PVEP影响较大,当刺激空间频率为0.1cpd时,对照组P1波潜伏期为(90.36±1.61)ms,实验组为(98.89±3.62)ms;空间频率0.3cpd时,对照组P1波潜伏期为(94.07±2.36)ms,实验组为(99.46±3.18)ms;与对照组比较,实验组潜伏期均有延长(t=23.00,P=0.001;t=9.26,P=0.016),其余空间频率差异不大。当空间频率大于等于0.5cpd时,实验组P1波振幅分别为:0.5cpd,(7.70±1.75)μV;0.6cpd,(7.52±2.01)μV;0.7cpd,(7.12±1.41)μV;0.8cpd,(7.65±1.69)μV;0.9cpd,(6.42±1.23)μV。对照组P1波振幅分别为:0.5cpd,(5.71±0.73)μV;0.6cpd,(5.15±0.20)μV;0.7cpd,(4.60±0.93)μV;0.8cpd,(4.65±0.83)μV;0.9cpd,(4.49±0.45)μV。两组比较,差异均有统计学意义(t=5.56、6.87、11.21、12.80、10.81,P<0.05)。激光对视力影响比较大,随着激光能量的增加,视力明显下降。结论植入式电极可以记录豚鼠PVEP图形,豚鼠的PVEP明显受到激光照射的影响,同时用PVEP评价动物的视力也是一种新方法。
Objective To study the effect of 680 nm-laser on pattern visual evoked potential （PVEP） of guinea pigs. Methods Twenty guinea pigs were divided into control group and experimental group （680 nm-laser, north electronic equipment research institute）, 10 in each group. After anesthesia, the guinea pigs＇scalp surface was exposed and two stainless screws （5 mm length and 1.2 mm diameter） were implanted at 6 mm in front of and 10 mm behind bregma, as reference electrode and recording electrode. In the middle, a third stainless screw was implanted as ground electrode. 3 days later, awaked guinea pigs were fixed in muhifunctional head-body apparatus. RETI-port system （Roland Consult, Germany） was used to record PVEP of control group and experiment group. Results The typical wave form was NPN in normal condition. The 680 nm-laser had an evident effect on PVEP. The latency of PI wave of experiment group was increased significantly [（98.89±3.62） ms and（99.46±3.18）ms] compared with control group （90.36±1.61） ms and （94.07±2.36）ms] when the spatial frequency was in 0.1 and 0.3 cpd （t=23.00, P=0.001, t=9.26, P=0.016）, but no significant in other spatial frequencies. The amplitude of P1 wave of experiment group[0.5 cpd, （7.70±1.75）μV; 0.6 cpd, （7.52±2.01）μV; 0.7 cpd, （7.12±1.41）μV; 0.8 cpd, （7.65±1.69）μV; 0.9 cpd, （6.42± 1.23）μV] was decreased significantly compared with control group [0.5 cpd, （5.71±0.73）μV; 0.6 cpd, （5.15±0.20）μV; 0.7 cpd, （4.60±0.93）μV; 0.8 cpd, （4.65±0.83）μV; 0.9 cpd, （4.49±0.45）μV] when the spatial frequency was beyond 0.5 cpd [（t=5.56, 6.87, 11.21, 12.80, 10.81, P〈0.05）]. Six hundred and eighty nm-laser had an obvious effect on visual acuity . The visual acuity decreased quickly with the laser energy increased. Conclusion Implanted electrode can record PVEP in awaked guinea pigs. The 680 nm- laser has an evident effect on PVEP. Meanwhile evaluation of animal visual acuity using PVEP is a good method
出处
《眼视光学杂志》
2009年第4期241-245,共5页
Chinese Journal of Optometry & Ophthalmology
基金
国家自然科学基金项目(30571999)
