摘要
试验研究了利用秦党茎段进行组织培养的方法,结果表明:在光照强度1600 lx,温度25℃的条件下,秦党幼茎诱导愈伤组织及芽较快的初代培养基是MS+2,4-D0.5 mg.L-1+蔗糖20 g.L-1+琼脂8 g.L-1及MS+BA2.0 mg.L-1+IAA1.0 mg.L-1+蔗糖20 g.L-1+琼脂8 g.L-1;继代培养基为MS+BA1.5 mg.L-1+NAA0.1 mg.L-1+蔗糖30 g.L-1+琼脂8 g.L-1直接分化形成幼苗,或MS+IBA1.0 mg.L-1+蔗糖30 g.L-1+琼脂8 g.L-1诱导形成无根苗再进行生根培养。
The culture technique of stem segments of Codonopsis tsinlingensis was studied in the experiment. The results showed when the intensity of illumination was 1600 1x and the temperature was 25℃, to the young stem, the initial medium as MS ＋ 2,4 -D 0.5 mg·L^-1 ＋ sucrose 20 g·L^-1 ＋ agar 8 g·L^-1 and MS ＋ BA 2.0 mg·L^-1 ＋ IAA 1.0mg·L^-1 ＋ sucrose 20 g·L^-1 ＋ agar 8 g·L^-1 could induce callus and bud more quickly, the subculture medium as MS ＋ BA1.5 mg·L^-1 ＋ NAA 0.1mg·L^-11 ＋ sucrose 30 g·L^-1 ＋ agar 8 g·L^-1 could differentiate seedling directly or as MS ＋ IBA1.0 mg·L^-1 ＋ sucrose 30 g·L^-1 ＋ agar 8 g·L^-1 could induce rootless seedling and then rooted culture.
基金
安康学院专项科研计划资助项目(2005AZX012)
关键词
秦党
茎段培养
愈伤组织
分化
Codonopsis tsinlingensis
stem segment culture
callus
differentiation
