摘要
猪戊型肝炎是一种人畜共患病，为了异清上海地区猪群中戊型肝炎的流行情况，评估其感染人的风险，于不同季节调查上海地区不同区（县）规模化猪场中不同月龄猪戊型肝炎病毒的感染状况。试验应用逆转录-巢式聚合酶链法（RT-nPCR）对9个区（县）51个猪场（次）的猪粪便标本和1个生猪屠宰中心的127份猪胆汁标本进行HEV RNA检测，结果为51个猪场次中31场次为阳性，阳性率为60．78％。1699份猪粪便标本中201份为HEV RNA阳性，阳性率为11．83％；阳性标本季节分布明显，以夏秋季为主，平均阳性率为14．63％，而冬春季平均阳性率为5．91％；1月龄以内的仔猪感染率低于10％，为8．60％，其他均在10％～15％左右；9个养猪的区（县）中均存在戊型肝炎病毒的感染，但差异也较大，最高为18．30％，而低的只有0．61％。127份猪胆汁标本中有9份为阳性，阳性率为7．09％。结果说明上海地区的猪群中存在着较为广泛的猪戊型肝炎病毒的感染，提示密切接触者存在感染的风险。
In order to study the infect situation of swine HEV in different seasones, pig farms and ages in Shanghai area. The PCR method have been uesed in the study. Dtected HEV RNA of 1 699 faces samples from 51 pig farms in Shanghai 9 diffferent areas and 127 bile samples from a slaughterhouse. The results showed that there were 31 postive in 51 pig farms, the positive rate was 60.78%. In all 1 699 faces samples, 201 samples were positive, the positive rate was 11.83%, in which summer and autumn＇s average positive rate （14.63%） was higher than spring and winter＇s （5.91%）, the pig under one month old positive rate was 8.60% which lower than others those positive rate aboout 10%-15%. 9 areas were all infected, but the positive rate was great difference, the highest rate was 18.30% and the most low rate was 0.61%. There were 9 postive in 127 bile samples, the positive rate was 7.09%. The conclusion was that swine HEV widely infected in Shanghai area and pepole close contacted were infectious risk.
出处
《上海交通大学学报（农业科学版）》
2009年第4期346-349,共4页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市科技兴农攻项目[沪农科攻字(2006第5-7号)]
