摘要
目的研究凹顶藻萜类化合物(Laurencia terpenoid extract,LET)对接种H_(22)瘤细胞小鼠白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平的影响。方法1.以反相高效液相色谱法测定LET中总萜的含量;2.昆明小鼠随机分为5组(10只/组),即模型组、LET低中高剂量组(25,50,100mg·kg^(-1)·d^(-1))、环磷酰胺组(CY对照组)。建立小鼠肝癌H_(22)移植性肿瘤模型,次日除模型组外,其余4组分别以不同剂量的LET、CY灌胃,共15d后处死,准确称取瘤重,计算抑瘤率,并采用放免法(RIA)测定血清中IL-6、TNF-α的水平。结果LET灌胃给药小鼠H_(22)肿瘤质量增长较模型组缓慢,抑瘤率分别为27.5%、34.9%和41.4%;且能升高细胞因子IL-6、TNF-α的水平。结论LET能较好地抑制H_(22)小鼠肿瘤增长,其抗肿瘤机制可能与升高机体细胞因子IL-6、TNF-α水平有关。
Objective To study the effects of Laurencia terpenoids（LET）on the levels of IL-6 and TNF-α in H22 mice. Methods 1. The content of total terpenoids in LET was detected by RP-HPLC. 2. The mice were randomly divided into five groups： the model group, the LET groups（25,50,100mg· kg^-1 · d^-1） and the Cyelophosphamide（CY） group, and transplant tumor model ofhepatoma 22 （H22） was established. The next day four groups of animal （except the model group） were treated with different dosage of LET and CY （ig） and executed after 15 days. The tumors were weighed and the tumor inhibition rates were calculated. Furthermore tile levels of IL- 6 and TNF-α in serum were determined by RIA. Results In vivv, the increases of H22 tumor weight in LET groups were slower than that in model group. The tumor inhibition rates were 27.5% ,34.9% and 41.4% correspondently. Mid and high-dose groups of I.ET could increase the levels of IL-6 and TNF-α. Conclusion LET could inhibit the increase of H22 tumor weight. The mechanism may be related to enhancing the level of cytokine including IL-6 and TNF-α.
出处
《中国海洋药物》
CAS
CSCD
2009年第4期9-12,共4页
Chinese Journal of Marine Drugs
基金
山东省科技厅科技攻关资助项目(2006GG2302002)
青岛市科技局科技计划资助项目(08-2-1-5-nsh)
