摘要
聚羟基脂肪酸酯(PHA)是一种具有广阔应用前景的生物可降解塑料,活性污泥中存在大量PHA合成菌,通过好氧/微好氧/沉淀的方式对污泥进行驯化,使PHA占污泥MLVSS的比例提高到21.19%。用BOX-PCR、ERIC-PCR和REP-PCR指纹图谱技术,对驯化前后污泥样品的菌群组成进行比较分析,结合尼罗蓝荧光平板法和菌落原位杂交技术,筛选得到活性污泥中PHA合成的优势菌株CAMP11。分类鉴定表明CAMP11属于芽孢杆菌属,FT-IR与NMR碳谱分析表明其合成的产物为羟基丁酸与羟基戊酸的共聚物(PHBV)。将菌株CAMP11回注入污泥驯化液,可使PHA占污泥MLVSS的比例进一步提高到32.08%。
Polyhydroxyalkanoates （PHA） are biodegradable thermoplastics with attractive application prospects. Many bacteria in activated sludge have the capacity to synthesize PHA, but the proportion of PHA-synthesis-bacteria with total microbe is very small. The numbers of PHA-synthesis-bacteria increased under the domestication of aerobic-anoxic- sedimentary condition, with the percents of PHA/MLVSS increasing to 21.19%. Differences of microbial communities between native sludge and domesticated sludge were analyzed with the method of BOX-PCR, ERIC-PCR and REP-PCR fingerprinting. Results of in situ colony hydridization and nile blue screening showed that strain CMAP11 which was identified as Bacillus sp. was the dominant PHA-synthesis-bacteria, and the structure of its product was PHBV by the method of FT-IR and ^13C-NMR, The percents of PHA/MLVSS increased to 32.08% after strain CAMPll were added into sludge at the initial stages of domestication.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2009年第9期124-128,共5页
Environmental Science & Technology
基金
天津市高等学校科技发展基金(20050808)
关键词
聚羟基脂肪酸酯
污泥驯化
DNA指纹图谱
优势菌
polyhydroxyalkanoates （PHA）
sludge domestication
DNA fingerprint
dominant bacteria
