摘要
有关厌氧消化中间产物的毒性研究,特别是有关多种中间产物的联合毒性研究,迄今鲜见文献报道。采用发光细菌法测定了4种典型厌氧消化中间产物乙醇、乙酸、丙酸和丁酸的急性毒性(15min-IC50)。结果表明,乙醇、乙酸、丙酸和丁酸对发光杆菌的半抑制浓度IC50分别为27.1、3.96、2.49和6.00g·L-1,其毒性大小顺序为:丙酸>乙酸>丁酸>乙醇。非解离态VFA是其主要抑制形态,非解离态乙酸、丙酸和丁酸的IC50值分别为23.4、19和37mg·L-1,相当于汞IC50值的234倍、190倍和370倍。4种厌氧消化中间产物对发光细菌的联合毒性作用方式皆为相加作用。研究结果为厌氧消化技术的调控和优化提供了理论依据。
Few researches on individual toxicity of anaerobic digestion intermediates have so far been reported, let alone researches on their combined toxicity. Ethanol, acetate, propionate and butyrate are four typical intermediates in anaerobic digestion and their acute toxicity are determined with Photobacterium phosphoreum. The results showed that the IC50 （half inhibition concentration for 15 min） values of ethanol, acetate, propionate and butyrate were 27.0, 3.96, 2.49 and 6.00 g.L^-1, respectively. The toxicity descended in the order of propionate, acetate, butyrate and ethanol. The inhibition of VFAs（ volatile fatty acids） in their undissociated forms was stronger than that in their dissociated forms. The IC50 values of undissociated acetic acid, propionic acid and butyric acid were 23.4, 19, 37 mg.L^-1, respectively, which were 234, 190, 370 times of the IC50 values of standard toxicity reference substrate HgCl2. The combined effects of ethanol and acetate, ethanol and pro- pionate, ethanol and butyrate, acetate and propionate, acetate and butyrate, propionate and butyrate were all additive effects. The combined effects of the four anaerobic digestion intermediates were also additive effects. The achievement has laid a basis for control and optimization of anaerobic biotechnology.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2009年第8期1651-1654,共4页
Journal of Agro-Environment Science
基金
国家863项目(2006AA06ZZ332)
浙江省重大科技攻关项目(2005C13004)
关键词
发光细菌
单一毒性
联合毒性
Photobacterium phosphoreum
single toxicity
combined toxicity
