摘要
目的:观察基泰对慢性乙型肝炎(CHB)患者树突状细胞(DC)生物学性状的影响。方法:应用密度梯度离心法分离CHB患者外周血单个核细胞,经rhGM-CSF和IL-4诱导3d后,分组培养。对照组不加基泰,实验组加入不同浓度的基泰(25、50、100、200、400mg/L),培养8d后,观察DC形态学变化,流式细胞仪测CD1a、CD80的表达,ELISA法检测培养上清液中IL-12含量,MTT法测定DC对同种异体淋巴细胞的增殖刺激指数(SI)。结果:倒置显微镜观察,实验组细胞表现出典型的DC形态。6组DCCD80、CD1a、IL-2分泌量及SI比较,差异均有统计学意义(F分别为902.26,1381.54,827.31,41.54,P均<0.05)。与对照组比较,100mg/L基泰组的DCCD1a和CD80表达增高(P<0.05),其上清液中IL-12水平增高(P<0.05),对同种异体淋巴细胞的SI增大(P<0.05)。结论:基泰一定程度上可增强CHB的DC免疫学活性。
Aim ： To investigate the effects of Jitai on the function of dendritic cell（DC） derived from patients with chronic hepatitis B （CHB）. Methods：Peripheral mononuelear cells of patients with CHB were isolated by density gradient centrifugation,and cultured with IL-4/GM-CSF for 3 d, then cultured with different eoncentreations of Jitai （25,50,100, 200,400 rag/L） to be the experimental groups. DC cultured withont Jitai were conducted as the control group. 8 d later,DC morphology was observed under light microscopy, CD80 and CD1 a were assayed by flow eytometry,and the concentration of IL-12 in the supernatant were assayed by ELISA,T cell proliferation induced by DC was assayed by MTT method. Results： The experimental group shared the gypical DC morphology. There were significant differences in expressions of CD80, CDla,the concentration of IL-12 in the supernatant, and SI on T cell proliferation among the 6 groups （F was 902.26, 1381.54,827.31,41.54, P 〈 0.05 ）. Compared with those of the control group, the secretion of IL-12 increased, the expressions of CD1 a,CD80, and SI of 100 mg/L Jitai group were significantly higher （ P 〈 0.05 ）. Conclusion ： Jitai in certain concentration could enhance the immunology function of DC derived from CHB.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2009年第4期814-816,共3页
Journal of Zhengzhou University(Medical Sciences)
