摘要
目的:观察肾上腺素(E)对离体豚鼠左心室流出道组织自发慢反应电位的影响。方法:采用标准玻璃微电极细胞内记录技术,观察E对离体豚鼠左心室流出道组织自发慢反应电位的影响。观测指标有:最大舒张电位(MDP),动作电位幅度(APA),0期最大除极速度(Vmax),4期自动除极速度(VDD),自发放电频率(RPF),复极50%和90%时间(APD50、APD90)。结果:用100μmol/LE灌流,豚鼠左心室流出道自律组织动作电位APA明显增大(P<0.01),Vmax明显增快(P<0.05),MDP显著增大(P<0.05),APD90显著缩短(P<0.01);RPF明显增快(P<0.01),VDD明显加快(P<0.05)。结论:E对左心室流出道组织自律细胞有兴奋作用。
Objective： To explore the effects of epinephrine（E）on spontaneous slow action potentials （APs）of guinea-pig left ventricular outflow tract cells. Methods： By using standard intracellular microeleetrode teehnique to record APs, E was used to investigate the electrophysiological features of left ventricular outflow tract cells. Electrophysiological parameters examined were： maximal diastolic potential（MDP）, amplitude of ac- tion potential（APA）, maximal rate of depolarization （Vmax）, velocity of diastolic depolarization （VDD）, rate of pacemaker firing（RPF）,50%and 90% of duration of action potential（APDs0 and APD90 ）. Results：The results obtained were as follows：Application of 100μmol/I. E resulted in a significant increase in VDD（P〈0.05）, RPF （P〈0.01） ,APA（P〈0. 05）,MDP（P〈0.05）and Vmax（P〈0. 05） ;a notable shorteness in and APD90 （P〈0. 01）in the pacemaker cells. Conclusion. These results suggested that E should excite the pacemaker cells of left ventrieular outflow tract.
出处
《河北北方学院学报（医学版）》
2009年第4期4-6,共3页
Journal of Hebei North University:Medical Edition
基金
河北省教育厅自然科学研究项目(编号:2005312)
关键词
肾上腺素
电生理学技术/心脏
豚鼠
左心室流出通
自律细胞
Epinephrine, Electrophysiology Techniques/Cardiac, Guinea Pigs, Left Ventricular out-flow Tract. Pacemaker Cells
