摘要
为探讨白藜芦醇在肿瘤细胞中的结合靶点蛋白质。采用亲和甄别磁珠法生物淘洗白藜芦醇的靶点蛋白质。在构建并优化了白藜芦醇结构模型的基础上,通过分子动力学优化分析白藜芦醇与其靶点蛋白质的结构模型,并且使用分子对接分析验证两者的结合作用。结果表明,亲和甄X别磁珠法直接筛选到的能与白藜芦醇的特异性结合的蛋白质是Myosin蛋白质和Actin蛋白质,并且成功构建得到了合理的白藜芦醇分子与Actin蛋白质的复合物的三维结构。通过分析白藜芦醇分子与Ac-tin蛋白活性氨基酸残基结合模式发现,残基Val30,Phe31,Pro32,Thr203,Ala204,Glu205,Pro243,Asp244,等对两者的结合都有重要贡献。白藜芦醇是通过作用于肿瘤细胞的骨架结构蛋白来干扰细胞的有丝分裂过程,从而导致肿瘤细胞的体外增殖受到抑制。
It was to study the binding proteins of resveratrol in cancer cell. The affinity beads analysis was confirmed to identify the binding protein of the resveratrol on the cell of MCF-7. At first, the structure model of resveratrol was built and optimized. The binding effect between them was verifieated and analyzed by docking. It was showed directly that myosin protein and aetin protein were the specific binding-protein of the resveratrol on the cell of MCF-7. The binding pattern of resveratrol with active residues of actin protein was also analyzed, and the result showed that several residues including Val30, Phe31, Pro32, Thr203, Ala204, Glu205, Pro243 and Asp244 have important effect on their interactions. The anticanccr mechanism of resveratrol may be due to the fact that it could induce the intervention of the cell mitosis through interaction with the proteins of eystoskeleton.
出处
《生物技术通报》
CAS
CSCD
北大核心
2009年第8期128-133,共6页
Biotechnology Bulletin
基金
国家自然科学基金资助项目(30772586)
