摘要
目的:探讨系统性红斑狼疮(SLE)患者血清中共刺激分子sCD40L、转化生长因子TGF-β1的测定对疾病活动性的预测价值。方法:采用酶联免疫吸附试验(ELISA)检测SLE患者和正常人血清sCD40L和TGF-β1的水平,分析两种指标的含量与SLE患者的疾病活动度指数SLEDAI和免疫学指标(ANA,ds-DNA等)的相关性,并进行SLE病例的前瞻性和治疗前后对照研究。结果:(1)SLE患者血清sCD40L水平为5.87μg/L,显著高于正常对照组2.31μg/L;而SLE患者血清TGF-β1为48.3μg/L,显著低于正常对照组70.4μg/L。(2)SLE患者血清sCD40L水平活动期高于稳定期(P<0.05),ANA高滴度组高于ANA低滴度组,ds-DNA抗体阳性组高于ds-DNA抗体阴性组,并与SLE患者SLEDAI呈正相关(r=0.253);SLE患者血清TGF-β1水平活动期与稳定期差异无统计学意义(P>0.05),与SLE患者SLEDAI亦无明显相关(r=-0.071)。(3)以SLE非活动性组的x-±s作为评判临界值,定量检测sCD40L对SLE活动性的预测具有较好的灵敏度、特异度。结论:sCD40L与SLE发病有关,可反映SLE患者的疾病活动性,定量检测可有助于预测患者的病情活动和转归。
Objective： To study the serum sCD40L and TGF-β1 correlating with the value of therapeutic prediction of the disease activity in systemic lupus erythematosus （SLE）. Methods： Mensurated Serum sCD40L and TGF-β1 levels in patients with SLE and healthy controls were detected by using a commercially available ELISA system and the correlation of the levels of two detecting indexes in SLE patients were analyzed with SLEDAI scores and other immune markers （ ANA, ds-DNA etc） . The judgment of disease activity with the levels of sCD40L and TGF-β1 was also studied. Results ： （ 1 ） The average level of sCD40L in SLE patients was 5.87 μg/L, higher than 2.31 μg/L in the healthy controls. The average level of TGF-β1 in SLE patients was 48.3 μg/L, lower than 70.4 μg/L in the healthy controls. （2）Serum level of sCD40L was significantly higher in active SLE patients than those in inactive patients （P 〈 0.05 ）, serum level of sCD40L was significantly higher in patients with higher concentration of ANA, positive ds-DNA antibody than in patients with lower concentration of ANA, negative ds-DNA antibody. The serological level of sCD40L was shown to have a positive correlation with SLEDAI scores （ r = 0.253 ）. There was no difference between serum level of TGF-β1 in active SLE patients and serum level of TGF-β1 in inactive SLE patients（P 〉0.05）. The level of TGF-β1 was shown to have no correlation with SLEDAI scores（r = -0.071 ） . （3）The average level x^- ＋ s of inactive SLE patients was used as the critical value, the determination of serum sCD40L has higher sensitivities and specificities to the value of therapeutic prediction. Conclusion： sCD40L might play certain roles in the pathogenesis of SLE, and might be correlated with the disease activity. The determination of serum sCD40L has the value of therapeutic prediction in systemic lupus erythematosus.
出处
《江苏大学学报（医学版）》
CAS
2009年第4期328-332,共5页
Journal of Jiangsu University：Medicine Edition
基金
苏州市科技计划资助项目(SZD0790)
