摘要
目的研究血清对体外培养条件下二十二碳六稀酸(DHA)抑癌作用的影响及DHA的抗癌机制。方法采用MTT法观察血清刺激和无血清情况下DHA对体外培养的肺癌A549细胞的生长抑制作用,同时采用免疫印迹方法检测DHA对血清诱导的蛋白激酶B(Akt)磷酸化的影响。结果在无血清和低浓度血清(<2%)下,DHA明显抑制A549细胞的生长;在高浓度血清下,DHA则呈现促进A549生长的作用。无血清下,DHA对血清诱导的Akt的活化具有抑制作用。结论体外培养条件下,培养基中无血清时DHA可能通过脂质过氧化降低Akt的活化、抑制A549细胞的生长;高血清含量则明显干扰DHA的抗癌活性。
Objective To study the effects of serum on anticancer activity of DHA and its antitumor mechanism. Method The growth inhibition of DHA on lung cancer A549 cells, and the effect of DHA on serum-induced protein kinase B （Akt） activation were assessed by MTT and Western blot respectively. Results DHA markedly induced growth inhibition of A549 cells in low concentration of serum, but promoted growth of A549 cells in high concentration of serum. DHA significantly blocked serum-induced activation of Akt in serum-free medium. Conclusion Serum is obviously against DHA-mediated growth inhibition of A549 cells, and DHA inhibits activation of Akt by lipid peroxidation and blocks growth of A549 ceils.
出处
《营养学报》
CAS
CSCD
北大核心
2009年第4期322-324,329,共4页
Acta Nutrimenta Sinica
基金
博士点基金
关键词
DHA
血清
AKT
生长抑制
DHA
serum
protein kinase B
growth inhibition
