摘要
目的观察高磷条件体外培养的原代大鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)是否发生钙含量的变化及其电镜下的微观表现。方法应用大鼠原代血管平滑肌细胞进行体外实验,将培养细胞分为对照组和高磷组。在培养的10d内观察细胞的形态学变化,并应用原子分光光度计检测细胞钙含量,应用电镜观察细胞的微观变化。结果对照组细胞VSMCs钙含量在10d内无明显增加,高磷组VSMCs钙含量在培养第8天后明显增加,与换液当天比较差异有统计学意义(P<0.01)。2组细胞电镜下第10天VSMCs细胞外基质中均可见大量胶原纤维,高磷组还可见高密度电子致密物呈颗粒状沿胶原纤维沉积。结论高磷条件可诱导体外培养的大鼠VSMCs细胞钙含量增加。电镜下高密度电子致密物呈颗粒状并沿胶原纤维沉积。
Objective To investigate the effect of high phosphate on calcification of rat vascular smooth muscle cells （VSMCs） and the morphological features of calcified rat＇s VSMCs under transmission electron microscope. Methods VSMCs from explants of rat aortic media were cultured. Cells were divided into two groups： the normal calcium（Ca） and phosphate group（ control group, phosphate 1.4 mmol/L, Ca 2.0 mmol/L） , and high phosphate group（phosphate 2.4 mmoL/L, Ca 2.0 mmol/L）. Calcium deposition of cells were measured by atomic absorptiometry. VSMCs of both groups cultured for 10 days were examined under transmission electron microscope. Results After ten days culture, the calcium deposition in high phosphate group increased significantly （ P 〈 0.01 ）. In contrast, there was little calcium deposition in control group. Collagen fibers were found in the extracellular matrix in both groups. Electron-dense calcified deposits were observed on the collagen fibers in high phosphate group. Conclusion This study suggests that elevated phosphate may directly stimulate rat VSMCs to be calcified. Electron-dense calcified deposits were mainly observed on the collagen fibers.
出处
《首都医科大学学报》
CAS
北大核心
2009年第4期521-524,共4页
Journal of Capital Medical University
关键词
血管平滑肌细胞
高磷
血管钙化
电镜
vascular smooth muscle cells
high phosphate
vascular calcification
transmission electron microscope
