摘要
目的探讨慢性低氧低糖培养对新生大鼠海马神经元α-分泌酶活性及表达的影响。方法取新生24h的Wistar大鼠海马原代培养神经元并予以鉴定,培养第七天予以12h、24h、36h低氧低糖培养干预,MTT法检测海马神经元的活力,荧光法检测α、β-分泌酶活性,Western blot检测α-分泌酶蛋白表达水平。结果低氧低糖培养12小时组海马神经元α-分泌酶活性升高(P<0.01),低氧低糖培养24h、36h组α-分泌酶(TACE)活性无明显变化(P>0.05),低氧低糖培养12h、24h、36h组海马神经元β-分泌酶(BACE1)活性升高(P<0.05);低氧低糖培养12h、24h、36h组海马神经元α-分泌酶蛋白表达水平降低(P<0.05)。结论慢性低氧低糖培养降低新生大鼠海马神经元α-分泌酶的表达。
Objective To investigate tbe effect of chronic hypoxic and hypoglycemic culture on α-secretase activities and expression of rat hippocampal neurons. Methods Primary cultures of Wistar rat hippoeampal neurons were prepared for the following procedures. At 7th day after cells plating,hippocampal neurons were treated with hy- poxic and hypoglycemic euhure for 12h,24h,and 36h respectively. Cells vitality were assayed by MTT, fluorescence detection and Western blot were applied respectively to assay tx-seeretase expression and activity. Results The ac- tivity of tx-secretase was increased at 12h time point（ P 〈 0.01 ） and no significantly alterations at other time points（ P 〉 0.05 ） , β-secretase activity was increased at all time points （ P 〈 0.05 ） ; α-secretase expression was desereased at all time points after chronic hypoxic and hypoglycemic culture（P 〈 0.05）. Conclusion α-secretase expression and activity of rat hippocampal neurons changed after chronic hypoxic and hypoglycemie culture.
出处
《脑与神经疾病杂志》
2009年第2期141-144,共4页
Journal of Brain and Nervous Diseases
