摘要
牛活体采卵体外成熟后,常规进行体外受精,平均卵裂率为73.1%。在卵裂率稳定的前提下,随机挑选96枚没有卵裂的MⅡ期卵母细胞进行Hoechst33342染色分析,以期找出卵子没有分裂的原因,为进一步提高卵裂率提供科学资料。结果发现精子没有进入卵母细胞的占59.4%,精子进入卵母细胞但雌雄原核未融合的占19.8%,多精受精引起发育阻滞的占13.5%。结果表明,在保证卵母细胞成熟的前提下,引起牛体外受精失败的主要原因是精子没有进入卵母细胞。
Bovine oocytes obtained from OPU （ovum pick up ） were subjected to IVF （in vitro fertilization） and 96 oocytes at MⅡ stage but with IVF failure were selected to stain with Hoechst33342. The data showed that 57 oocytes （59.4%） were not observed the presence of male pronuclei, and the proportion which female and male nuclei did not fuse was 19.8% （19/96）, whilemulti-sperm entrance accounted for 13.5% （13/96）. The results suggested that the main reason of bovine IVF failure, on the precondition of oocyte maturation, was caused by the failure of sperm entrance.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第8期91-94,共4页
China Animal Husbandry & Veterinary Medicine
