摘要
【研究目的】对畜禽大肠杆菌具有抑制作用的动物饲用益生菌短短芽孢杆菌LPF-2的培养条件进行优化。【方法】以抑菌圈为指标,从时间、温度、摇床转速和培养基pH值研究短短芽孢杆菌LPF-2的最适培养条件,以获得短短芽孢杆菌LPF-2最佳的抑菌效果。【结果】短短芽孢杆菌LPF-2培养24～84h的抑菌圈为10.00～13.00mm,在24～39℃下培养的抑菌圈为9.00～14.00mm,转速在90～210r/min下培养的抑菌圈为13.75～15.00mm,pH6.0～8.0下培养的抑菌圈为10.50～14.50mm。【结论】动物益生菌短短芽孢杆菌LPF-2的最适培养条件为培养基pH7.5、培养温度30℃、培养时间48h,摇床转速在90～210r/min范围内均可。
[OBJECTIVE] The fermentation conditions were optimized for animal feed probiotics, BrevibaciUus brevis LPF-2, which strongly inhibited Escherichia coll. [METHOD]The best fermentation conditions for the highest inhibition of LPF-2 were studied based on time, temperature, shaking speed and medium pH value by using inhibition zones as an index. [RESULTS]The results showed that its inhibition zones against E. Coli were 10.00-13.00 mm after cultured for 24-84 h, 9.00-14.00 mm at 24-39 ℃, 13.75-15.00 mm at the shaking speeds of 90-210 r/min and 10.50-14.50 mm at pH values of 6.0-8.0, respectively. [CONCLUSION] n conclusion, the best fermentation conditions for LPF-2 against E. Coli were as follows： pH 7.5, 30 ℃, 48 h culture time and 90-210 r/min shaking speed.
出处
《中国农学通报》
CSCD
北大核心
2009年第16期13-16,共4页
Chinese Agricultural Science Bulletin
基金
国家科技部科技支撑计划"华南村镇塘坝地表饮用水安全保障适用技术研究与示范"(2008ZX07425-002)
福建省发展和改革委员会五新项目"远程监控微生物发酵舍零排放养猪法的研究与应用"
福建省农业科学院科技创新团队建设基金(STIF-Y03)
福建省发改委农业专项(闽发改投资[2008]762号)
关键词
培养条件
芽孢杆菌
大肠杆菌
抑制
益生菌
fermentation conditions, Brevibacillus brevis, Escherichia coli, inhibition, probiotics
