摘要
研究了催化光度法测定茶叶中硒（Ⅳ）的催化反应影响因素。结果表明：在用间苯二胺偶联剂的催化显色体系中，加硒（Ⅳ）催化反应条件各因素对吸光度（A）的影响，随催化条件不同而发生变化。其影响的主要因素是缓冲液（KCl－HC，pH0．50）用量，应严格控制；其次是氧化剂（KClO3，0．6mol／L）用量，以及反应温度、还原剂（盐酸苯肼0．02mol／L）用量、反应时间和偶联剂用量。
The factors of catalytic reaction for determination of selenium (Ⅳ) in tea with catalytic spectropho-tometric analysis were studied. The results showed that in the catalytic reaction system using pheylendiamine as coupling agent, the effects of various catalytic reaction conditions with selenium (Ⅳ) on light absorption (A) were changed with the catalytic conditions. The most important factor was the amount of buffer (KCl-HCl, pH0.50), which should be strictly controlled, followed in the order of the amount of oxidizer (KClO3,0.6 mol/L), the reaction temperature. the amount of reducing agent (phenylhydrazine hydrochloride, 0. 02 mol/L), the duration of reaction and the amount of coupling agent.
出处
《茶叶科学》
CAS
CSCD
1998年第1期70-73,共4页
Journal of Tea Science
关键词
催化光度法
催化条件
茶叶
硒
Catalytic spectrophotometry Catalytic conditions Tea Selenium
