摘要
研究山楂中熊果酸提取分离的工艺条件,以熊果酸浸出率为指标,通过正交试验考察各因素对熊果酸浸出率的影响,经乙醇提取和大孔吸附树脂分离纯化得到熊果酸。优化的提取工艺条件为提取溶剂90%乙醇、固液比1:10(m/V)、提取时间2.5h、提取温度80℃,熊果酸浸出率达1.07%。AB-8大孔吸附树脂分离纯化的最优条件为样品溶液熊果酸浓度3.01～4.01mg/ml、洗脱剂pH10.0,依次用水、不同体积分数的乙醇以1.5ml/min的流速洗脱,收集乙醇洗脱液,熊果酸回收率为98.1%,纯度为97.0%。结果表明,该提取工艺的产品纯度高、得率高,可适合于工业化的生产。
This study investigated the ethanol extraction and the purification using macroporous adsorption resin of ursolic acid from hawthorn fruits. An orthogonal array design involving 16 experiments of four crucial factors at four levels was employed to maximize the ethanol extraction. The optimal macroporous adsorption resin and solvent for purifying ursolic acid were determined and the dynamic adsorption and desorption of ursolic acid on macroporous adsorption resin AB-8 were investigated. The optimum extraction conditions of ursolic acid were as follows： using 90% ethanol as the extractant and a ratio of solid to liquid of 1：10, extraction at 80 ℃ for 2.5 h, and the extraction yield of ursolic acid was 1.07% under these conditions. The optimum purification conditions with macroporous adsorption resin AB-8 were as follows： ursolic acid concentration 3.01 - 4.01 mg/mi and eluent pH 10.0 for a stepwise elution with water and different concentrations of ethanol solution at a flow rate of 1.5 ml/min. A purity of 97.0% and a recovery of 98.1% were achieved under the optimized purification conditions. An ursolic acid product with high purity and yield can be obtained by this extraction and purification process and therefore it is a promising approach for the industrialized production of ursolic acid.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第16期177-180,共4页
Food Science
基金
广州市教育系统创新学术团队项目(200915)
关键词
山楂
分离纯化
熊果酸
大孔吸附树脂
hawthorn
separation and purification
ursolic acid
macroporous adsorption resin
