摘要
目的研究不同医院分离的鲍曼不动杆菌产碳青霉烯酶的基因型和分子流行特征。方法采用琼脂稀释法检测64株多重耐药鲍曼不动杆菌对抗菌药物的最小抑菌浓度（MIC）；PCR检测碳青霉烯酶基因、整合酶基因及per基因，选取不同的耐药克隆菌株进行序列测定；脉冲场凝胶电泳（PFGE）分析菌株同源关系，确定菌株的分子流行特征。结果50株菌（78．1％）携带blaOXA-23-like基因，经测序分析确定为OXA．23；1株菌检测出blaOXA-58-like基因；57株菌（89．1％）携带I类整合子；25株菌（39．1％）检测出blaPER-1基因。PFGE图谱显示64株菌分为A、B、C、D、E等13个基因型，三家医院分别以A型、B型和U型为主要流行株。结论三家医院均发现多耐药鲍曼不动杆菌的播散流行，不同医院间和同一医院不同科室间存在同型别流行；三家医院多耐药鲍曼不动杆菌产生的碳青霉烯酶常携带OXA-23型酶，同时检测出blaOXA-58、I类整合子和blaFER基因。
Objective To investigate antibiotic resistance, carbapenemase genotype and the molecular epidemiology of multidrug-resistant Acinetobacter baumannii （Aba） collected from 3 military hospitals in China. Methods The minimum inhibitory concentrations（MIC） were examined by ager dilution method. Genotypes of carbapenemases were amplified by multiplex PCR and its products were sequenced. PCR was used to detect per gene. Homology of the resistant isolates was analyzed by pulse-field gel electrophoresis （PFGE）. Results Among the 64 MDRA strains, 78.1% （50） strains possessed blaOXA-23 gene, 89.1%（57） carried Class 1 integrase gene, 39.1%（25） with blaPER-1 gene, and 1 strain with blaOXA-58-like gene. PFGE showed that 13 （A, B, C, D, E genotype） different clones were identified in these strains. A, B, and U clones were the predominant clones in three hospitals, respectitively. Conclusion Outbreaks of multidrug-resistant Aba occurred at 3 military hospitals with the most prevalent carbapenemase as OXA-23 enzyme. OXA-58 type of carbapenemase and per-1 in Aba were also isolated.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2009年第8期832-835,共4页
Chinese Journal of Epidemiology
基金
军队“十一五”课题（06MA294）
关键词
鲍曼不动杆菌
多重耐药
分子流行病学
Acinetobacter baumannii
Multidrug-resistant
Epidemiology, molecular
